林业科学 ›› 2021, Vol. 57 ›› Issue (1): 20-29.doi: 10.11707/j.1001-7488.20210103
闫海冰,张慧芳,冯帆,于兆友,杨秀清*
收稿日期:
2019-10-30
出版日期:
2021-01-01
发布日期:
2021-03-10
通讯作者:
杨秀清
基金资助:
Haibing Yan,Huifang Zhang,Fan Feng,Zhaoyou Yu,Xiuqing Yang*
Received:
2019-10-30
Online:
2021-01-01
Published:
2021-03-10
Contact:
Xiuqing Yang
摘要:
目的: 研究盐胁迫下2种白刺叶片代谢物及其代谢通路的变化,以揭示白刺对盐胁迫的代谢响应机制,为有效提高白刺对盐碱地的持续生物改良及适应性研究提供理论依据。方法: 对唐古特白刺和西伯利亚白刺实生苗进行300 mmol·L-1 NaCl胁迫处理,蒸馏水处理为对照,采用GC-TOF-MS代谢组学方法,分析盐胁迫对2种白刺叶片代谢物及其代谢通路的影响。结果: 1) 与未胁迫处理相比,盐胁迫后唐古特白刺叶片存在差异代谢物11种,其中8种显著上调,包括1种脂肪酸、5种有机酸、1种醇和1种碱基衍生物;而西伯利亚白刺叶片中存在差异代谢物108种,其中106种显著上调,包括51种氨基酸、22种糖、11种脂肪酸、8种有机酸、7种醇、5种生物碱以及2种维生素。2)通过KEGG注释可将唐古特白刺的差异代谢物富集到6条代谢通路中,拓扑分析筛选到与代谢物差异相关性最高的关键路径为硫代谢、TCA循环及二羧酸循环;而西伯利亚白刺的差异代谢物可被富集到46条代谢通路中,其中,氨基酸代谢多达13条,筛选到的关键路径为缬氨酸、亮氨酸和异亮氨酸的生物合成、C5支链二元酸代谢、泛酸和CoA生物合成。结论: 2种白刺对盐胁迫有不同的代谢响应机制。唐古特白刺通过以有机酸为主的11种代谢物参与盐胁迫响应,并通过加强以硫代谢为主的6条代谢通路来应答盐胁迫。西伯利亚白刺通过以氨基酸、糖、脂肪酸为主的108种代谢物参与盐胁迫响应,并通过加强以氨基酸代谢为主的46条代谢通路应答盐胁迫。西伯利亚白刺参与盐胁迫调控的代谢物较唐古特白刺种类更多,代谢途径更广,对盐胁迫的代谢响应更为显著。
中图分类号:
闫海冰,张慧芳,冯帆,于兆友,杨秀清. 2种白刺对盐胁迫的代谢响应机制[J]. 林业科学, 2021, 57(1): 20-29.
Haibing Yan,Huifang Zhang,Fan Feng,Zhaoyou Yu,Xiuqing Yang. Metabolic Response Mechanism of Two Nitraria Species to Salt Stress[J]. Scientia Silvae Sinicae, 2021, 57(1): 20-29.
表1
盐胁迫后2种白刺叶片中的差异代谢物"
序号 No. | 唐古特白刺差异代谢物 Differentially expressed metabolites of N. tangutorum | 上下调倍数 Up-down multiple | 西伯利亚白刺差异代谢物 Differentially expressed metabolites of N. sibirica | 上下调倍数 Up-down multiple |
1 | O-琥珀酰-L-高丝氨酸O-Succinylhomoserine | 2.68 | 缩二脲Biuret | 14.45 |
2 | 亚油酸Linoleic acid | 2.36 | (2R, 3S)-2-羟基-3-异丙基丁二酸(2R, 3S)-2-hydroxy-3-isopropylbutanedioic acid | 13.80 |
3 | 柠檬酸Citric acid | 2.13 | N-甲基-DL-丙氨酸N-Methyl-DL-alanine | 4.14 |
4 | 3, 4-二羟基肉桂酸3, 4-dihydroxycinnamic acid | 1.85 | 磷酸甲酯Methyl phosphate | 4.08 |
5 | 酒石酸Tartaric acid | 1.82 | 谷氨酸Glutamic acid | 3.68 |
6 | 金鸡纳酸Quinic acid | 1.43 | 6-磷酸葡萄糖酸6-phosphogluconic acid | 3.61 |
7 | 肌醇Myo-inositol | 1.40 | 2, 6-二磷酸果糖脱胶剂Fructose 2, 6-biphosphate degr prod | 3.42 |
8 | 葡庚糖酸Glucoheptonic acid | 1.32 | 5′-甲基硫代腺苷5′-methylthioadenosine | 3.21 |
9 | 胸腺嘧啶Thymine | -1.38 | 氧脯氨酸Oxoproline | 3.20 |
10 | 乳糖Lactose | -1.46 | 麦芽糖醇Maltitol | 3.16 |
11 | 2-氨基乙硫醇2-aminoethanethiol | -6.37 | 阿魏酸Ferulic acid | 3.14 |
12 | — | 缬氨酸Valine | 3.12 | |
13 | — | 丙氨酸Alanine | 3.12 | |
…… | — | …… | ||
106 | — | 6-羟基己酸二聚体6-hydroxy caproic acid dimer | 0.54 | |
107 | — | 黄素腺嘌呤衍生物flavin adenine degrad product | -0.49 | |
108 | — | ε-己内酰胺epsilon-Caprolactam | -11.95 |
表2
盐胁迫后2种白刺差异代谢物映射的KEGG通路"
种名 Specific name | 代谢通路(差异代谢物个数) KEGG pathway(Quantity of differential metabolites) | 代谢通路(差异代谢物个数) KEGG pathway(Quantity of differential metabolites) |
唐古特白刺 N. tangutorum | 代谢通路Metabolic pathways(7) | 次生代谢产物的生物合成Biosynthesis of secondary metabolites(2) |
半乳糖代谢Galactose metabolism(2) | 氨基酸生物合成Biosynthesis of amino acids(2) | |
二羧酸代谢Glyoxylate and dicarboxylate metabolism(2) | ABC转运ABC transporters(2) | |
西伯利亚白刺 N. sibirica | 代谢通路Metabolic pathways(42) | β-丙氨酸代谢beta-Alanine metabolism(3) |
次生代谢产物的生物合成Biosynthesis of secondary metabolites(20) | 牛磺酸与亚牛磺酸代谢Taurine and hypotaurine metabolism(3) | |
氨基酸生物合成Biosynthesis of amino acids(9) | 丙酸盐代谢Propanoate metabolism(3) | |
环戊酮-2-羧酸甲酯代谢2-Oxocarboxylic acid metabolism(7) | 硫代谢Sulfur metabolism(3) | |
ABC转运ABC transporters(7) | 芥子油苷的生物合成Glucosinolate biosynthesis(3) | |
碳代谢Carbon metabolism(6) | 脂肪酸降解Fatty acid degradation(2) | |
苯丙氨酸代谢Phenylalanine metabolism(6) | 三羧酸循环Citrate cycle(2) | |
氰胺酸代谢Cyanoamino acid metabolism(6) | 糖酵解/糖异生Glycolysis/ Gluconeogenesis(2) | |
氨酰-tRNA生物合成Aminoacyl-tRNA biosynthesis(6) | 半乳糖代谢Galactose metabolism(2) | |
缬氨酸、亮氨酸及异亮氨酸的生物合成Valine, leucine and isoleucine biosynthesis(6) | 戊糖和葡萄糖醛酸的相互转化Pentose and glucuronate interconversions(2) | |
托烷、哌啶和吡啶生物碱的生物合成Tropane, piperidine and pyridine alkaloid biosynthesis(5) | 泛醌和其他萜类醌的生物合成Ubiquinone and other terpenoid-quinone biosynthesis(2) | |
泛酸和辅酶A生物合成Pantothenate and CoA biosynthesis(5) | 氧化磷酸化Oxidative phosphorylation(2) | |
半胱氨酸和甲硫氨酸代谢Cysteine and methionine metabolism(5) | 嘌呤代谢Purine metabolism(2) | |
精氨酸与脯氨酸代谢Arginine and proline metabolism(4) | 单杆菌胺生物合成Monobactam biosynthesis(2) | |
络氨酸代谢Tyrosine metabolism(4) | 谷胱甘肽代谢Glutathione metabolism(2) | |
二羧酸盐代谢Glyoxylate and dicarboxylate metabolism(4) | 甘油酯代谢Glycerolipid metabolism(2) | |
丁酸代谢Butanoate metabolism(4) | 丙酮酸代谢Pyruvate metabolism(2) | |
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