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林业科学 ›› 2019, Vol. 55 ›› Issue (1): 22-30.doi: 10.11707/j.1001-7488.20190103

• 论文与研究报告 • 上一篇    下一篇

荧光假单胞菌与红绒盖牛肝菌共接种对杨树根际土壤酶活性及微生物多样性的影响

刘辉1,2, 吴小芹1, 任嘉红1, 陈丹1   

  1. 1. 南京林业大学南方现代林业协同创新中心 南京林业大学林学院 南京 210037;
    2. 安徽师范大学环境科学与工程学院 芜湖 241003
  • 收稿日期:2017-09-11 修回日期:2018-09-30 出版日期:2019-01-25 发布日期:2019-01-06
  • 基金资助:
    国家重点研发计划资助项目(2017YFD0600104);国家林业公益性行业科研专项(201004061);江苏高校优势学科建设工程资助项目(PAPD);国家自然科学基金项目(31400589)。

Effect of Co-Inoculation with Pseudomonas fluorescens and Xerocomus chrysenteron on the Soil Enzyme Activity and Microbial Diversity in Poplar Rhizosphere

Liu Hui1,2, Wu Xiaoqin1, Ren Jiahong1, Chen Dan1   

  1. 1. Co-Innovation Center for Sustainable Forestry in Southern China College of Forestry, Nanjing Forestry University Nanjing 210037;
    2. College of Environmental Science and Engineering, Anhui Normal University Wuhu 241003
  • Received:2017-09-11 Revised:2018-09-30 Online:2019-01-25 Published:2019-01-06

摘要: [目的]探究荧光假单胞菌与红绒盖牛肝菌共接种条件下NL-895杨根际土壤酶活性和土壤微生物功能多样性的变化,从微生态水平揭示溶磷细菌与外生菌根真菌互作对NL-895杨的促生机制。[方法]采用盆栽试验考察荧光假单胞菌JW-JS1与红绒盖牛肝菌(Xc)共接种后NL-895杨根际土壤酶(酸性磷酸酶、脱氢酶和转化酶)的活性变化,并通过BIOLOG微孔板法分析共接种对NL-895杨根际土壤微生物群落和功能多样性的影响。[结果]从整体趋势来看,不同接种处理后NL-895杨根际土壤酶活性均有所增强,土壤酸性磷酸酶、脱氢酶和转化酶活性均大于对照,其中,共接种JW-JS1+Xc处理对土壤酸性磷酸酶和转化酶活性的促进最为明显;接种150天后,不同接种处理土壤微生物的AWCD值均高于CK(培养24~48 h除外),且随着培养时间的延长呈先上升后稳定的趋势;培养96 h后,共接种JW-JS1+Xc处理土壤微生物McIntosh优势度指数和碳源利用丰富度指数显著高于单接种Xc或JW-JS1和CK(P<0.05),而Shannon丰富度指数和Simpson优势度指数显著低于单接种Xc或JW-JS1和CK(P<0.05);不同接种处理NL-895杨根际土壤微生物对BIOLOG微孔板中6大类碳源的利用能力差异显著(P<0.05)。[结论]荧光假单胞菌JW-JS1与外生菌根真菌Xc共接种对NL-895杨根际土壤酶活性和土壤微生物功能多样性的提高具有一定的促进作用,有利于其土壤肥力的改善,进而促进NL-895杨的生长。与单接种相比,溶磷细菌与外生菌根真菌共接种可更有效地提高杨树根际土壤酶活性和土壤微生物多样性。

关键词: 荧光假单胞菌, 红绒盖牛肝菌, 共接种, 土壤酶活性, BIOLOG微孔板法, 微生物多样性

Abstract: [Objective] Under co-inoculation with Pseudomonas fluorescens JW-JS1 and Xerocomus chrysenteron, the changes of enzyme activity and the microbial functional diversity in rhizosphere soil of NL-895 Poplar were studied to reveal the interaction mechanism by co-inoculation with phosphate-solubilizing bacteria and ectomycorrhizal fungi at the micro-ecological level.[Method] A pot experiment was conducted to investigate the effects of co-inoculation with Pseudomonas fluorescens JW-JS1 and Xerocomus chrysenteron(Xc) on the activities of soil enzymes (acid phosphatase, dehydrogenase and invertase), and the BIOLOG microplate method was applied to assay the microbial community and its function diversity in rhizosphere soil of NL-895 Poplar.[Result] The results showed that the soil enzymes activities (acid phosphatase, dehydrogenase and invertase) in rhizosphere of NL-895 Poplar with different inoculation were all higher than those of control, especially for acid phosphatase and invertase when co-inoculation with JW-JS1 and Xc. The BIOLOG plates analysis showed that AWCD values on day 150 after inoculation were generally higher than those of control (except for 24 hour and 48 hour), and the value first increased then stabilized with the prolongation of incubation time. After 96 hour incubation, the McIntosh index and Richness index of co-inoculation with JW-JS1+Xc were higher than single inoculation with Xc or JW-JS1 and those of control (P<0.05), but the Shannon-Wiener index and Simpson index were significantly lower (P<0.05). There was significant difference in the utilization ability of six carbon sources in BIOLOG microplates by soil microorganism in rhizosphere of NL-895 Poplar with different inoculation treatments (P<0.05).[Conclusion] The co-inoculation of Pseudomonas fluorescens JW-JS1 and Xerocomus chrysenteron may play roles in improving the activities of soil enzymes and enriching soil microbial communities. All of these are conductive to improve soil fertility and promote the growth of NL-895 poplar. The results also showed that co-inoculation with PSB + ECF could effectively increase the activity of soil enzymes and improve soil biodiversity compared with single inoculation with PSB or ECF, which could provide a theoretical basis for developing the compound microbial fertilizer in the future.

Key words: Pseudomonas fluorescens, Xerocomus chrysenteron, co-inoculation, soil enzyme activity, BIOLOG microplate, diversity of microbial community

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