一株荧光假单胞菌的溶磷特性及其对杨树的促生效果

林业科学 ›› 2013, Vol. 49 ›› Issue (9): 112-118.

一株荧光假单胞菌的溶磷特性及其对杨树的促生效果

刘辉^1,2, 吴小芹¹, 任嘉红¹, 陈丹¹

1. 南京林业大学森林资源与环境学院南京 210037;
2. 安徽师范大学环境科学与工程学院芜湖 241003

收稿日期:2012-09-02 修回日期:2013-04-15 出版日期:2013-09-25 发布日期:2013-09-19
通讯作者: 吴小芹
基金资助:
国家林业公益性行业科研专项(201004061);江苏省重大科技支撑与自主创新专项(BE2008393);江苏省研究生培养创新工程项目(CX08B_028Z)。

Phosphate-Dissolving Characteristics and Growth Promoting Effect of Pseudomonads fluorescent JW-JSI on Poplar Seedlings

Liu Hui^1,2, Wu Xiaoqin¹, Ren Jiahong¹, Chen Dan¹

1. College of Forest Resources and Environment, Nanjing Forestry University Nanjing 210037;
2. College of Environmental Science and Engineering, Anhui Normal University Wuhu 241003

Received:2012-09-02 Revised:2013-04-15 Online:2013-09-25 Published:2013-09-19

摘要/Abstract

摘要：

从杨树根际筛选出一株荧光假单胞菌JW-JS1菌株,通过液体发酵试验研究其溶磷特性,并利用温室盆栽试验探究其对杨树苗木的促生效果。结果表明:该菌株分别以葡萄糖为碳源,硫酸铵为氮源和碳氮比为100∶1时溶磷能力最强,溶磷量分别达到580.72,708.34和608.97mg·L^-1;该菌株分别在温度为30℃,初始pH7.0,装液量为1/5和NaCl质量浓度为0时溶磷能力最强,溶磷量分别为562.89,594.58,638.62mg·L^-1和527.82mg·L^-1。接种该菌株可显著促进NL-895杨和美洲黑杨苗期的生长和营养元素含量的提高,其中,NL-895杨和美洲黑杨的苗高、地径、鲜质量和干质量分别比CK增长21.96%,5.04%,57.33%,54.36%和51.66%,1.98%,150.18%,168.49%,植株体内P,K,Ca和Mg含量分别比CK增长25.16%,19.75%,17.12%,10.10%和27.55%,125.53%,55.47%,102.94%。

关键词: 杨树, 荧光假单胞菌, 特性, 促生长

Abstract:

A phosphate-dissolving bacterium, Pseudomonads fluorescent JW-JS1, was selected from the poplar rhizosphere. The phosphate-dissolving characteristics of this isolated strain were studied by using liquid fermentation, and its growth promoting effect on poplar seedlings was conducted in a greenhouse. The results showed that the strain displayed its highest phosphate-dissolving capacity when using glucose as C source, (NH₄)₂SO₄ as N source and C/N as 100:1, and could dissolve phosphate at a rate of 580.72, 708.34 and 608.97 mg·L^-1, respectively; Under the following conditions of temperature 30 ℃, initial pH 7.0, filling amount 1/5 and 0% NaCl concentration, the strain produced the highest phosphate-dissolving capacity, and the phosphate-dissolving rate was as much as 562.89, 594.58, 638.62 and 527.82 mg·L^-1, respectively. The JW-JS1 significantly improved the growth of seedlings of NL-895 and P. deltoides, and could also increased the content of P, K, Ca and Mg in plants. As a result, the seedling height, diameter, fresh weight and dry weight of NL-895 and P. deltoides increased 21.96%, 5.04%, 57.33%, 54.36% and 51.66%, 1.98%, 150.18%, 168.49% than the control group, respectively; The contents of P, K, Ca and Mg in plants increased 25.16%, 19.75%, 17.12%, 10.10% and 27.55%, 125.53%, 55.47%, 102.94% than the control group, respectively.

Key words: poplar, Pseudomonads fluorescent, characteristics, promoting effect

中图分类号:

S718.7

刘辉, 吴小芹, 任嘉红, 陈丹. 一株荧光假单胞菌的溶磷特性及其对杨树的促生效果[J]. 林业科学, 2013, 49(9): 112-118.

Liu Hui, Wu Xiaoqin, Ren Jiahong, Chen Dan. Phosphate-Dissolving Characteristics and Growth Promoting Effect of Pseudomonads fluorescent JW-JSI on Poplar Seedlings[J]. Scientia Silvae Sinicae, 2013, 49(9): 112-118.

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