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林业科学 ›› 2013, Vol. 49 ›› Issue (7): 48-56.doi: 10.11707/j.1001-7488.20130707

• 论文与研究报告 • 上一篇    下一篇

接种ERM真菌对桃叶杜鹃幼苗的促生效应及生理生化影响

欧静1, 韦小丽1, 何跃军1, 刘仁阳1, 陈训2   

  1. 1. 贵州大学林学院 贵阳 550025;
    2. 贵州省科学院 贵阳 550001
  • 收稿日期:2013-01-14 修回日期:2013-02-23 出版日期:2013-07-25 发布日期:2013-07-23
  • 通讯作者: 陈训
  • 基金资助:

    贵州省农业科技攻关项目(黔科合NY字 3076号)。

Effects of Inoculation with ERM Fungi Isolates on the Growth and Physio-biochemical Properties of Rhododendron annae seedlings

Ou Jing1, Wei Xiaoli1, He Yuejun1, Liu Renyang1, Chen Xun2   

  1. 1. Forest College, Guizhou University Guiyang 550025;
    2. Guizhou Academy of Sciences Guiyang 550001
  • Received:2013-01-14 Revised:2013-02-23 Online:2013-07-25 Published:2013-07-23

摘要:

通过12个不同ERM真菌菌株接种2年生无菌实生桃叶杜鹃幼苗,研究接种对幼苗生长势与生物量的影响,分析ERM菌株对内源激素含量与氧化酶活性的变化。结果表明: 接种显著提高桃叶杜鹃幼苗的苗高、地径、叶面积、主根长和生物量。接种苗苗高比对照增加29.47%~73.68%,地径比对照增加11.16%~70.09%, 总生物量比对照增长3.86%~61.22%;地上部分干质量增幅最高值为99.69%,地下部分干质量增幅最高值为27.27%。ERM菌株显著提高接种苗地上和地下部分吲哚乙酸(IAA)、赤霉素(GA3)、玉米素核苷(ZR)、脱落酸(ABA)含量和超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性,降低了丙二醛(MDA)含量。ERM真菌可能是通过分泌激素或刺激植株分泌激素,几种激素物质的协调配合来促进桃叶杜鹃幼苗的生长。从综合接种效应来看,TY29,TY35,TY12,TY18,TY14和TY02是培育桃叶杜鹃菌根苗优良备选菌株。

关键词: ERM真菌, 促生效应, 内源激素, 氧化酶活性, 桃叶杜鹃

Abstract:

Twelve ericoid mycorrhiza (ERM) fungal isolates were used to inoculate 2-year-old Rhododendron annae Franch. seedlings to study effects of the inoculations on the plant growth potential, biomass and endogenous hormones contents and enzyme activity. The results showed that the inoculations with ERM significantly increased height, diameter, leaf area, mainroot length and biomass of the seedlings. The height increased by 29.47%-73.68% than that control, and diameter increased by 11.16%~70.09%. The total biomass increased by 3.86%-61.22%, of which the highest increase in shoot dry weight was 99.69%, and and the highest increase in underground part dry weight was 27.27%. The contents of indole acetic acid(IAA), gibberellin (GA3), zeatin riboside (ZR), abscisic acid (ABA) and the activity of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) in the inoculated seedlings with ERM were significantly higher than those in non-inoculated plants, while the malondialdehyde (MDA) content was less than that of control. ERM fungi might promote the growth of R. annae seedlings through secreting hormone or stimulating the secretion of plant hormones, or coordinating several hormones. As for the comprehensive effects of the inoculations, strains TY35, TY18, TY29, TY19, TY02, and TY12 were selected as optimum ERM isolates for seedlings of R. annae.

Key words: ericoid mycorrhiza fungi, growth promoting effects, endogenous hormone, oxidase activity, Rhododendron annae

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