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林业科学 ›› 2010, Vol. 46 ›› Issue (5): 58-63.doi: 10.11707/j.1001-7488.20100510

• 论文 • 上一篇    下一篇

多拷贝rol基因对转基因杨树生长及内源激素的影响

郝宇1,3,梁海永2,杨敏生2   

  1. 1.河北农业大学园林与旅游学院保定071000;2.河北农业大学林学院保定071000;3.天津国土资源和房屋职业学院艺术设计系 天津300270
  • 收稿日期:2009-03-30 修回日期:1900-01-01 出版日期:2010-05-25 发布日期:2010-05-25
  • 通讯作者: 杨敏生

Effect of the Multi-Copied Gene on the Growth and the Endogenous Hormones of the Transgenic Poplar

Hao Yu1,3;Liang Haiyong2;Yang Minsheng2   

  1. 1.College of Landscape and Travel,Agricultural University of HebeiBaoding 071000; 2.College of Forestry,Agricultural University of HebeiBaoding 071000;3.The Art Department of Design,Tianjin Land Resources and House Vocational CollegeTianjin 300270
  • Received:2009-03-30 Revised:1900-01-01 Online:2010-05-25 Published:2010-05-25

摘要:

对转入多拷贝rolB、rolC基因的三倍体毛白杨进行高生长量、生根率和内源激素(IAA、ABA)含量等指标的测定,以研究多拷贝rol基因在转基因植物中的表达。结果显示: 转Ri质粒三倍体毛白杨再次分别转入rolB、rolC基因后,部分株系试管苗的生长受到了抑制,但各株系生根率均有不同程度提高;rolB基因转化植株的内源IAA平均含量高于rolC基因转化植株,其IAA/ABA值亦高于rolC基因转化植株。试验结果表明rol基因的多拷贝促使转化植株快速大量生成毛状根。

关键词: rol基因, 三倍体毛白杨, 生长, 内源激素

Abstract:

In order to study the expression of multiple rol genes,height-growth,rate of rooting,endogenous hormone (IAA,ABA) and other parameters of the triploid Populus tomentosa were measured. The results indicated that growth of some transgenic plants was inhibited while the rooting rates of all the transgenic plants were increased to various degrees. Compared with the transgenic plants with rolC gene,the plants transferred with rolB gene were higher in both the IAA content and the rates of IAA/ABA on average. The results showed that the plants with multiple copies of rol gene were able to generate a large number of hairy roots quickly.

Key words: rolgene, triploid Populus tomentosa, growth, endogenous hormone