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林业科学 ›› 2009, Vol. 12 ›› Issue (9): 147-150.doi: 10.11707/j.1001-7488.20090925

• 论文 • 上一篇    下一篇

与地被菊株型匍匐性连锁RAPD标记的SCAR转化*

赵静媛 陈素梅 陈发棣   

  1. 南京农业大学园艺学院 南京 210095
  • 收稿日期:2008-07-04 修回日期:1900-01-01 出版日期:2009-09-25 发布日期:2009-09-25
  • 通讯作者: 陈发棣
  • 基金资助:
     

Conversion of RAPD Marker Linked to Creep Plant Type in Ground-Cover Chrysanthemum to SCAR Marker

Zhao Jingyuan,Chen Sumei,Chen Fadi   

  1. College of Horticulture, Nanjing Agricultural University Nanjing 210095
  • Received:2008-07-04 Revised:1900-01-01 Online:2009-09-25 Published:2009-09-25
  • Supported by:
     

摘要:  

关键词: 地被菊, 株型匍匐性, SCAR标记

Abstract:

In this study, PCR product amplified with a RAPD marker A10555 which is linked to gene controlling creeping habit of ground-cover chrysanthemum(Dendranthema grandiflorum)was recovered, and subsequently subcloned and sequenced. A pair of 18-base specific primers was designed based on the obtained sequence.The validity of this SCAR marker was verified by screening parents and 152 individuals of the F1 progeny, and the consistence in the locus of the special band and the number of recombinants was observed between SCAR analysis and RAPD analysis. The size of this SCAR marker was 555 bp, which was designated as SCA10555. The results demonstrated that the RAPD marker was converted to SCAR marker successfully. This study provides bases for new cultivars developing,molecular markers assisted breeding and creeping habit related genes cloning of the ground-cover chrysanthemum.

Key words: ground-cover chrysanthemum(Dendranthema grandiflorum), creeping, SCAR marker

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