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林业科学 ›› 2009, Vol. 12 ›› Issue (3): 145-149.doi: 10.11707/j.1001-7488.20090325

• 论文 • 上一篇    下一篇

毛竹捕光叶绿素a/b结合蛋白基因 cab PhE1 的克隆与表达分析

高志民1 刘成2 刘颖丽1 彭镇华3   

  1. (1. 国际竹藤网络中心 国家林业局竹藤科学与技术重点开放实验室 北京100102;2. 中国科学院植物研究所 北京100093; 3. 中国林业科学研究院林业研究所 北京100091)
  • 收稿日期:2008-03-13 修回日期:1900-01-01 出版日期:2009-03-25 发布日期:2009-03-25

Cloning and High Prokaryotic Expression of the Light Harvesting Chlorophyll a/bBinding Protein Gene from Bamboo(Phyllostachys edulis)

Gao Zhimin1,Liu Cheng2,Liu Yingli1,Peng Zhenhua3   

  1. (1. Key Laboratory of Bamboo and Rattan Science and Technology of State Forestry Administration International Center for Bamboo and Rattan Beijing 100102; 2.Institute of Botany, the Chinese Academy of Sciences Beijing 100093; 3. Research Institute of Forestry, Chinese Academy of Forestry Beijing 100091)
  • Received:2008-03-13 Revised:1900-01-01 Online:2009-03-25 Published:2009-03-25

关键词: 关键词: 毛竹, 捕光叶绿素a/b结合蛋白基因, 组织特异性表达, 原核表达

Abstract:

Abstract: The light harvesting chlorophyll a/bbinding protein is one of key proteins in the transformation from light energy to chemical energy. An open reading frame coding precursor protein of cab gene was cloned from the first strand of bamboo cDNA through RTPCR methods, and named as cabPhE1 (cab gene 1 from Phyllostachys edulis EF207229). The sequence analysis showed that the deduced polypeptide was highly homologous to some other CAB proteins from monocotyledon, and the gene belonged to lhcb2 family. Tissue specific expression showed that cabPhE1expressed higher in leaf than sheath and stem. The prokaryotic expression vector of cabPhE1gene encoding the mature protein was constructed by subcloning the fragment into pET23 a and was expressed in Escherichia coli induced by IPTG. The molecular weight of the induced protein was about 28 ku,approximate to that of the mature protein. This work is a key to the further research on in vitro reconstitution of lightharvesting Chl a/b complexes.

Key words: Key words: bamboo(Phyllostachys edulis), light harvesting chlorophyll a/bbinding protein gene(cab), tissue specific expression, prokaryotic expression