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林业科学 ›› 2009, Vol. 12 ›› Issue (3): 150-154.doi: 10.11707/j.1001-7488.20090326

• 论文 • 上一篇    下一篇

四倍体油茶的鉴定及变异

李铁柱1,2 田大伦1,2 乌云塔娜1,3 谭晓风1,3 项文化1,4 闫文德1,5     

  1. (1.中南林业科技大学 长沙410004; 2.南方林业生态应用技术国家工程实验室 长沙410004;3.经济林育种与栽培国家林业局重点实验室 长沙410004; 4.湖南会同杉木林生态系统国家野外科学观测研究站 会同418300;5.城市森林生态湖南省重点实验室 长沙410004)
  • 收稿日期:2008-09-26 修回日期:1900-01-01 出版日期:2009-03-25 发布日期:2009-03-25

Identification and Variation of Tetraploid Camellia oleifera

Li Tiezhu1,2,Tian Dalun1,2,Wuyun Tana1,3,Tan Xiaofeng1,3,Xiang Wenhua1,4,Yan Wende1,5   

  • Received:2008-09-26 Revised:1900-01-01 Online:2009-03-25 Published:2009-03-25

摘要:

(1.Central South University of Forestry and Technology Changsha 410004; 2.National Engineering Laboratory for Applied Technology of Forestry & Ecology in South China Changsha 410004; 3. Key Laboratory of NonWood Forest Product of State Forestry AdministrationChangsha 410004; 4.Huitong Ecological Station for Chinese Fir Plantation of Hunan Province Huitong 418300;5.Key Laboratory of Urban Forest Ecology of Hunan Province Changsha 410004)

关键词: 关键词: 油茶, 四倍体, 倍性鉴定, 诱变育种

Abstract:

Abstract: In this study, tetraploid of oil camellia (Camellia oleifera) was identified from the superior clones which were treated with colchicine, and the tetraploid variation was investigated with the experimental material. Analysis of the number of chromosome showed the chromosome number of the tetraploid oil camellia was 2n=4x=60, compared with the diploid (2n=2x=30). The morphological observation showed that the leaf area, leaf length, leaf width, leaf thickness and the space of leaf in the tetraploid were respectively increased by 98%, 385%, 25%, 4825%, 67%, compared with the diploid. The chlorophyll content and the length and width of guard cells were respectively increased by 4787%, 4815% and 3726%, while the leaf\|shape index reduced 381% than that in the control group. In addition, the leaf of tetraploid plant became more round, the total DNA content was twice as the control group was, and the net photosynthetic rate was also greater than that of the control group. The genetic polymorphism analysis with the mt DNA and cpDNA primers showed that no polymorphic locus was found in the tetraploid, which meant that the tetraploid was autotetraploid, however the genetic polymorphism analysis in the nDNA exhibited that the polymorphic ratio was 51%.

Key words: Key words: Camellia oleifera, tetraploid, chromosome identification, mutation breeding