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›› 1989, Vol. 25 ›› Issue (4): 317-322.

• 论文及研究报告 • 上一篇    下一篇

日本赤松毛虫质型多角体病毒Dendrolimus Spectabilis CPV对马尾松毛虫的生物测定

陈昌洁 王志贤 刘革   

  1. 中国林业科学研究院林业研究所
  • 收稿日期:1988-02-29 修回日期:1900-01-01 出版日期:1989-07-25 发布日期:1989-07-25

BIOASSAY OF JDS-CPV ON THE PINE CATERPILLAR DENDROLIMUS PUNCTATUS

Chen Changjie,Wang Zhixian,Liu Ge   

  1. Forest Research Institute, Chinese Academy of Forestry
  • Received:1988-02-29 Revised:1900-01-01 Online:1989-07-25 Published:1989-07-25

摘要: 应用日本赤松毛虫质型多角体病毒(以下简称JDS-CPV)对马尾松毛虫第一代3龄和5龄幼虫进行了生物测定。结果表明,JDS-CPV对3龄和5龄幼虫的LC(50)分别为1.6×105CPB/ml和3.3×105CPB/ml。其回归直线方程分别为Y=2.44+0.49xY=1.83+0.57x。对一代3龄虫的LD(50)为2.1×103CPB/ml,回归直线方程为Y=1.72+0.98x。以1×108CPB/ml浓度为例的LT(50),3龄虫为8.5天,5龄虫为13天。证实JDS-CPV对我国马尾松毛虫有较好的毒力。

关键词: 松毛虫, 质型多角体病毒, 生物测定

Abstract: Bioassay of JDS-CPV was conducted on the 3rd instar and 5th instar larvae of pine caterpillar D. punctatus. The regression equations between concentration and mortality for 3rd instar and 5th instar were Y=2.44+0.49x and Y=1.83+0.57x, LC(50) value were 1.6×105PIB/mL and 3.3×105PIB/ml respectively. The regression equation between the count of PIB and mortality for 3rd instar larvae is Y=1.72×0.98x, LD(50) value is 2.1×103PIB/larvae. For example as 1×108PIB/ml, the LT(50) value for 3rd instar and 5th instar larvae were 8.5 days and 13 days respectively. The results showed that the JDS-CPV has higher virulence to pine caterpillar.

Key words: Pine caterpillar, Cytoplasmic Polyhedrasis Virus, Bioassay