林业科学 ›› 2022, Vol. 58 ›› Issue (3): 175-186.doi: 10.11707/j.1001-7488.20220319
• 研究简报 • 上一篇
李勇1,2,黄锦1,于翠1,莫荣利1,朱志贤1,董朝霞1,胡兴明1,庄楚雄2,邓文1,*
收稿日期:
2020-06-29
出版日期:
2022-03-25
发布日期:
2022-06-02
通讯作者:
邓文
基金资助:
Yong Li1,2,Jin Huang1,Cui Yu1,Rongli Mo1,Zhixian Zhu1,Zhaoxia Dong1,Xingming Hu1,Cuxiong Zhuang2,Wen Deng1,*
Received:
2020-06-29
Online:
2022-03-25
Published:
2022-06-02
Contact:
Wen Deng
摘要:
目的: 研究淹水胁迫下不同繁育方式桑树的叶片光合生理参数、渗透调节物质和保护酶系统的动态变化, 揭示不同繁育方式桑树对水淹逆境的响应策略及差异, 为桑树在消落带逆境的生态修复应用提供理论基础。方法: 选择嫁接、扦插繁殖的蜀果(SG)和澳玉(AY)桑苗, 通过套盆模拟水淹胁迫(ys), 在0、3、7、12、18天测定桑树叶片的光合参数、叶绿素含量、渗透调节物质含量(可溶性蛋白质、可溶性糖、脯氨酸、丙二醛)及叶片保护酶活性(超氧化物歧化酶、过氧化物酶、过氧化氢酶、抗坏血酸过氧化酶)的动态变化, 探讨不同繁育方式桑树对水淹逆境的响应差异。结果: 1) 叶绿素含量随水淹天数增加而下降, 但不同品种桑苗之间有差异(P>0.05); 2) CK处理叶片的净光合速率(Pn)日变化呈双峰曲线, 而ys处理的桑苗呈单峰曲线, 且扦插繁育的值大于嫁接繁育(P>0.05), 桑树叶片光合参数动态变化结果显示, 各处理桑树叶片、气孔导度(Gs)和蒸腾速率(Tr)随水淹胁迫时间的延长呈先下降后上升趋势, 且扦插繁育值大于嫁接繁育(P>0.05); 3) 随着水淹时间延长, 各处理桑树叶片渗透调节物质含量变化整体呈下降趋势; 4) 各处理桑树能在整个水淹胁迫期间通过增加超氧化物歧化酶(SOD)活性来清除活性氧, 维持生理生长, 但过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化酶(APX)的活性呈下降趋势。结论: 水淹胁迫降低了桑树的光合作用, 但QC繁育桑树的光合作用能力大于嫁接繁育的。随水淹时间延长, 2个品种桑树均不能通过积累渗透条件物质来降低渗透势。SOD为桑树应答水淹胁迫的主要防御酶。对不同品种的水淹胁迫响应速度, 扦插繁育的SG快于嫁接繁育的, 而嫁接繁育的AY快于扦插繁育的; 在本试验水淹逆境下, SG适宜扦插繁育而AY适宜嫁接繁育。
中图分类号:
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