杉木炭疽病拮抗菌HY32的发酵条件优化

doi:10.11707/j.1001-7488.20141213

林业科学 ›› 2014, Vol. 50 ›› Issue (12): 94-100.doi: 10.11707/j.1001-7488.20141213

杉木炭疽病拮抗菌HY32的发酵条件优化

李红军¹, 周国英¹, 路宗岩¹, 谭益民¹, 段爱国²

1. 中南林业科技大学林学院长沙 410004;
2. 中国林业科学研究院林业研究所北京 100091

收稿日期:2014-02-15 修回日期:2014-07-31 出版日期:2014-12-25 发布日期:2015-01-08
通讯作者: 周国英
基金资助:
国家林业公益性行业科研专项(201001014).

Optimization of Fermentation Medium Formula and Culture Conditions for the Antagonistic Bacterium HY32 against Colletotrichum gloeosporioides

Li Hongjun¹, Zhou Guoying¹, Lu Zongyan¹, Tan Yimin¹, Duan Aiguo²

1. College of Forestry, Central South University of Forestry and Technology Changsha 410004;
2. Research Institute of Forestry, CAF Beijing 100091

Received:2014-02-15 Revised:2014-07-31 Online:2014-12-25 Published:2015-01-08

摘要/Abstract

摘要：

采用单因素试验法和响应面法,探讨杉木炭疽病拮抗菌HY32发酵培养基及发酵条件的优化途径.结果表明:该菌株最佳发酵培养基的最适碳源为葡萄糖,最适氮源为酵母膏和蛋白胨; 最佳培养基配方为葡萄糖19.80 g ·L^-1、酵母膏12.16 g ·L^-1、蛋白胨4.22 g ·L^-1、NaCl13.13 g ·L^-1.该菌株最佳发酵培养条件为培养温度30.52 ℃、初始pH8.09、摇床转速184.67 r ·min^-1.验证结果表明采用响应面法优化菌株HY32的发酵培养基及发酵条件是可行的.

关键词: 杉木, 拮抗菌HY32, 发酵条件, 响应面法

Abstract:

Bacterium HY32 was identified as an antagonistic strain against Colletotrichum gloeosporioides previously. Both the fermentation medium formula and culture conditions for the antagonistic bacterium HY32 were optimized by using a single factor test and a response surface methodology (RSM) in this study. The test results indicated that the optimized carbon source was glucose, nitrogen source was yeast extract and peptone, and inorganic salt was NaCl. Based on the single facfor test, the optimized concentration of these four major media components was determined. The OD₆₀₀ (optical density at 600 nm) value of cultures after 48 h fermentation was measured to assess the fermentation rate. The analyzed data indicated that the optimal medium was as the following: glucose 19.80 g ·L^-1, yeast extract 12.16 g ·L^-1, peptone 4.22 g ·L^-1 and NaCl 13.13 g ·L^-1. With this optimal medium, we found that the actual OD₆₀₀ value of HY32 culture was able to reach a high level of 1.743 after 48 h fermentation. Single factor test results also showed that the initial pH value, culture temperature and rotation speed were key factors to influence the bacterial growth and inhibitory rate. The best culture conditions were determined as the following: culture temperature 30.52 ℃, pH 8.09 and rotation speed 184.67 r ·min^-1 respectively.

Key words: Cunninghamia lanceolata, antagonistic bacterium HY32, culture conditions, response surface methodology

中图分类号:

S763.15

李红军, 周国英, 路宗岩, 谭益民, 段爱国. 杉木炭疽病拮抗菌HY32的发酵条件优化[J]. 林业科学, 2014, 50(12): 94-100.

Li Hongjun, Zhou Guoying, Lu Zongyan, Tan Yimin, Duan Aiguo. Optimization of Fermentation Medium Formula and Culture Conditions for the Antagonistic Bacterium HY32 against Colletotrichum gloeosporioides[J]. Scientia Silvae Sinicae, 2014, 50(12): 94-100.

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杉木炭疽病拮抗菌HY32的发酵条件优化

Optimization of Fermentation Medium Formula and Culture Conditions for the Antagonistic Bacterium HY32 against Colletotrichum gloeosporioides

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