新疆药用植物黑桑的抗氧化性

doi:10.11707/j.1001-7488.20140808

林业科学 ›› 2014, Vol. 50 ›› Issue (8): 53-59.doi: 10.11707/j.1001-7488.20140808

新疆药用植物黑桑的抗氧化性

王传宏, 刘超, 刘静, 向伟, 黄先智, 徐立

西南大学生物技术学院重庆 400715

收稿日期:2013-10-14 修回日期:2014-06-05 出版日期:2014-08-25 发布日期:2014-07-31
基金资助:
The Fundamental Research Funds for the Central Universities(XDJK2013C053).

Antioxidant Activity of Medicine Mulberry (Morus nigra) in Xinjiang

Wang Chuanhong, Liu Chao, Liu Jing, Xiang Wei, Huang Xianzhi, Xu Li

College of Biotechnology, Southwest University Chongqing 400715

Received:2013-10-14 Revised:2014-06-05 Online:2014-08-25 Published:2014-07-31
Contact: 徐立
Supported by:
The Fundamental Research Funds for the Central Universities(XDJK2013C053).

摘要/Abstract

摘要： 为寻找一种天然的抗氧化材料，以新疆药用植物黑桑的根、枝、叶和果实的乙醇提取物为材料，进行总多酚、总黄酮、抗氧化能力以及螯合Fe²⁺测定。结果显示：果实（桑椹）提取物（EEMF）的总多酚含量（0.943 8 mg·mL^-1）以及总黄酮含量（0.174 7 mg·mL^-1）与叶提取物（EEML）、枝提取物（EEMB）和根提取物（EEMR）相比并不高，但EEMF的抗氧化能力最强，其清除羟基自由基、DPPH自由基能力分别达到96.46%，96.83%，抑制脂质体氧化能力达到71.14%；此外，EEMF螯合Fe²⁺能力也强于其他3种。表明黑桑4个部位都具有良好的抗氧化能力，且果实最佳。

关键词: 药桑, 黑桑, 总多酚, 总黄酮, 抗氧化性, 螯合Fe²⁺

Abstract: In order to find a natural antioxidant material, ethanolic extracts of fruits (EEMF), leaves (EEML), branches (EEMB) and roots (EEMR) of medicine mulberry(Morus nigra) were investigated. The results showed that although the medicine mulberry fruits did not have the highest total polyphenol content (0.943 8 mg·mL^-1) and total flavonoids content (0.174 7 mg·mL^-1) among those organs, the EEMF possessed the strongest antioxidant effect. The abilities of scavenging hydroxyl radicals, scavenging DPPH radicals and inhibiting lipid oxidation reached to 96.46%, 96.83% and 71.14%, respectively. In addition, Fe²⁺ chelating ability of EEMF was stronger than that of EEMR, EEMB and EEML. Therefore, medicine mulberry fruit can be used to develop a natural and nutritious food additive.

Key words: medicine mulberry, Morus nigra, total polyphenols, total flavonoids, antioxidant activity, Fe²⁺-chelating activity

中图分类号:

S718.43

王传宏, 刘超, 刘静, 向伟, 黄先智, 徐立. 新疆药用植物黑桑的抗氧化性[J]. 林业科学, 2014, 50(8): 53-59.

Wang Chuanhong, Liu Chao, Liu Jing, Xiang Wei, Huang Xianzhi, Xu Li. Antioxidant Activity of Medicine Mulberry (Morus nigra) in Xinjiang[J]. Scientia Silvae Sinicae, 2014, 50(8): 53-59.

参考文献

Bae S H, Suh H J. 2007. Antioxidant activities of five different mulberry cultivars in Korea. LWT-Food Sci Tech, 40(6): 955-962.
Birt D F, Hendrich S, Wang W, et al. 2001. Dietary agents in cancer prevention: flavonoids and isoflavonoids. Pharmacology and Therapeutics, 90(2/3): 157-177.
Bocco A, Cuvelier M E, Richard H, et al. 1998. Antioxidant activity and phenolic composition of citrus peel and seed extracts. Journal of Agricultural and Food Chemistry, 46(6): 2123-2129.
Botterweck A A M, Verhagen H, Goldbohm R A. 2000. Intake of butylated hydroxyanisole and butylated hydroxytoluene and stomach cancer risk: results from analyses in the Netherlands cohort study. Food and Chemical Toxicology, 38(7): 599-605.
Chang L W, Juang L J, Wang B S. 2011. Antioxidant and antityrosinase activity of mulberry (Morus alba L.) twigs and root bark. Food and Chemical Toxicology, 49(4): 785-790.
Cheung L M, Cheung P C K, Ooi V E C. 2003. Antioxidant activity and total phenolics of edible mushroom extracts. Food Chemistry, 81(2): 249-255.
Chen P N, Chu S C, Chiou H L. 2006. Mulberry anthocyanins, cyanidin 3-rutinoside and cyanidin 3-glucoside, exhibited an inhibitory effect on the migration and invasion of a human lung cancer cell line. Cancer Letters, 235(2): 248-259.
Chew Y L, Goh J K, Lim Y Y. 2009. Assessment of in vitro antioxidant capacity and polyphenolic composition of selected medicinal herbs from Leguminosae family in Peninsular Malaysia. Food Chem, 116(1): 13-18.
Chu Y H, Chang C L, Hsu H F. 2000. Flavonoid content of several vegetables and their antioxidant activity. Journal of the Science of Food and Agriculture, 80(5): 561-566.
David P T, Gerard M J. 1997. The use of endogenous antioxidants to improve photoprotection. Photochem Photobiol, 41 (1/2): 1-10.
Devasagayam T, Tilak J C, Boloor K K. 2004. Free radicals and antioxidants in human health: current status and future prospects. Assoc Physicians India, 52: 794-804.
Finkel T, Holbrook N J. 2000. Oxidants, oxidative stress and the biology of ageing. Nature, 408: 239-247.
Germanas J P, Wang S, Miner A, et al. 2007. Discovery of small-molecule inhibitors of tyrosinase. Bioorg Med Chem Lett, 24(17): 6871-6875.
Hairi B, Salle G, Andary C. 1991. Involvement of flavonoids in the resistance of two popular cultivars to mistletoe (Viscum album L.). Protoplasma, 162: 20-26.
Jeong J B, Seo E W, Jeong H J. 2009. Effect of extracts from pine needle against oxidative DNA damage and apoptosis induced by hydroxyl radical via antioxidant activity. Food and Chemical Toxicology, 47(8): 2135-2141.
Jiang Y(江岩). 2010. Extraction and determination of anthocyanins from Morus nigra L. growing in Xinjiang. Food Science(食品科学), 31(14): 93-96.
Kang T H, Hur J Y, Kim H B. 2006. Neuroprotective effects of the cyaniding-3-O-beta-d-glucopyranoside isolated from mulberry fruit against cerebral ischemia. Neuroscience Letters, 391: 122-126.
Krikorian R, Shidler M D, Nash T A, et al. 2010. Blueberry supplementation improves memory in older adults. J Agric Food Chem, 58(7): 3996-4000.
Lloyd R V, Hanna P M, Mason R P. 1997. The origin of the hydroxyl radical oxygen in the Fenton reaction. Free Radic Biol Med, 22(5): 885-888.
Maimaiti Yiming(买买提依明). 2007. Study on Xinjiang medicine mulberry. North Sericulture(北方蚕业), 28(1): 1-3.
Maimaiti Yiming(买买提依明), Guo H X(郭洪荣), Wu L L(吴丽莉). 2002. Xinjiang medicine mulberry fruits' pigment separation and nutrition analysis. China Sericulture(中国蚕业), 23(3): 15-16.
Peng C, Chan H Y E, Huang Y, et al. 2011. Apple polyphenols extend the mean lifespan of Drosophila melanogaster. J Agric Food Chem, 59(5): 2097-2106.
Peng C, Zuo Y Y, Kwan K M, et al. 2012. Blueberry extract prolongs lifespan of Drosophila melanogaster. Experimental Gerontology, 47(2): 170-178.
Proestos C, Boziaris I S, Nychas G J E, et al. 2006. Analysis of flavonoids and phenolic acids in Greek aromatic plants. Investigation of their antioxidant capacity and antimicrobial activity. Food Chem, 95(4): 664-671.
Sakanaka S, Tachibana Y, Okada Y. 2005. Preparation and antioxidant properties of extracts of Japanese persimmon leaf tea (kakinoha-cha). Food Chem, 89(4): 569-575.
Shukla S, Mehta A, Bajpai V K, et al. 2009. In vitro antioxidant activity and total phenolic content of ethanolic leaf extract of Stevia rebaudiana Bert. Food and Chemical Toxicology, 47 (9): 2338-2343.
Sies H. 1986. Biochemistry of oxidative stress. Angewandte Chemie International Edition in English, 25 (12): 1058-1071.
Socha R, Juszczak L, Pietrzyk S, et al. 2009. Antioxidant activity and phenolic composition of herbhoneys. Food Chem, 113(2): 568-574.
Stohs S J, Bagchi D. 1995.Oxidative mechanism in the toxicity of metal ions. Free Radic Biol Med, 18(2): 321-336.
Tamura H, Shibamoto T. 1991. Antioxidantive activity measurement and 4-hydroxy nonenal. Am Oil Chem Soc, 68: 941-943.
Tang S Z, Kerry J P, Sheehan D, et al. 2002. Antioxidative mechanisms of tea catechins in chicken meat systems. Food Chemistry, 76(1): 45-51.
Tsuda T, Horio F, Uchida K, et al. 2003. Dietary cyaniding 3-O-beta-D-glucoside rich purple corn color prevents obesity and ameliorates hyperglycemia in mice. J Nutr, 133: 2125-2130.
Wang B S, Chang L W, Yen W J, et al. 2007. Antioxidative effect of sesame coat on LDL oxidation and oxidative stress in macrophages. Food Chem, 102(1): 351-360.
Wang S Y, Jiao H. 2000. Scavenging capacity of berry crops on superoxide radicals, hydrogen peroxide, hydroxyl radicals, and singlet oxygen. J Agric Food Chem, 48:5677-5684.
Wolfe K, Wu X, Liu R H. 2003. Antioxidant activity of apple peels. Journal of Agricultural and Food Chemistry, 53(3): 609-614.
Yen G C, Chen H Y. 1995. Antioxidant activity of various tea extracts in relation to their antimutagenicity. Agric Food Chem, 43(1): 27-32.
Yusuf Y, Romeo T, Toledo J. 2004. Major flavonoids in grape seeds and skins: Antioxidant capacity of catechin, epicatechin, and gallic acid. Agric Food Chem, 52(2): 255-260.

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新疆药用植物黑桑的抗氧化性

Antioxidant Activity of Medicine Mulberry (Morus nigra) in Xinjiang

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