油茶果糖-1, 6-二磷酸醛缩酶基因(CoFBA4)的分子特征与表达分析

doi:10.11707/j.1001-7488.20131123

林业科学 ›› 2013, Vol. 49 ›› Issue (11): 164-170.doi: 10.11707/j.1001-7488.20131123

油茶果糖-1, 6-二磷酸醛缩酶基因(CoFBA4)的分子特征与表达分析

曾艳玲, 谭晓风, 蒋瑶, 刘敏, 王建勇, 周俊琴

中南林业科技大学森林培养与保护教育部重点实验室经济林育种与栽培国家林业局重点实验室长沙 410004

收稿日期:2013-04-03 修回日期:2013-06-29 出版日期:2013-11-25 发布日期:2013-11-26
通讯作者: 谭晓风
基金资助:
国家自然科学基金项目（31070603）；中南林业科技大学青年基金重点项目（QJ2011008A）。

Molecular Characterization and Expression Analysis of Fructose-1, 6-Diphosphate Aldolase Gene (CoFBA4) from Camellia oleifera

Zeng Yanling, Tan Xiaofeng, Jiang Yao, Liu Min, Wang Jianyong, Zhou Junqin

Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees of Ministry of Education Key Laboratory of Non-Wood Forest Products of State Forestry Administration Central South University of Forestry and Technology Changsha 410004

Received:2013-04-03 Revised:2013-06-29 Online:2013-11-25 Published:2013-11-26

摘要/Abstract

关键词: 油茶, 果糖-1, 6-二磷酸醛缩酶, 基因克隆, 基因表达, 亚细胞定位

Abstract:

Fructose-1,6-diphosphate aldolase (FBA) in plants is not only one of the key regulatory enzyme in glycolysis pathway but also provide substrate source for two key components of oil synthesis. In this paper, a new FBA full-length cDNA of Camellia oleifera seed was isolated and cloned by RACE technology, and was named CoFBA4 (GenBank number: JX914590). CoFBA4 had an open reading frame with 1 185 base pairs encoding 394 amino acids. Sequence analysis showed that CoFBA4 had nearest genetic distance with FBA of Arabidopsis thaliana, and belonged to subfamily A. CoFBA4 contained glycosylation and fructose-1,6-diphosphate aldolase activity site, but had no transmembrane domain. CoFBA4 belonged to hydrophilic protein. Subcellular localization analysis showed that CoFBA4 acted out of cell. There was a positive relationship between expression abundance of CoFBA4 gene and oil yield from different superior clones during the same maturity period.

Key words: Camellia oleifera, fructose-1,6-diphosphate aldolase, gene cloning, gene expression, subcellular localization

中图分类号:

S718.46

曾艳玲, 谭晓风, 蒋瑶, 刘敏, 王建勇, 周俊琴. 油茶果糖-1, 6-二磷酸醛缩酶基因(CoFBA4)的分子特征与表达分析[J]. 林业科学, 2013, 49(11): 164-170.

Zeng Yanling, Tan Xiaofeng, Jiang Yao, Liu Min, Wang Jianyong, Zhou Junqin. Molecular Characterization and Expression Analysis of Fructose-1, 6-Diphosphate Aldolase Gene (CoFBA4) from Camellia oleifera[J]. Scientia Silvae Sinicae, 2013, 49(11): 164-170.

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油茶果糖-1, 6-二磷酸醛缩酶基因(CoFBA4)的分子特征与表达分析

Molecular Characterization and Expression Analysis of Fructose-1, 6-Diphosphate Aldolase Gene (CoFBA4) from Camellia oleifera

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