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林业科学 ›› 2012, Vol. 48 ›› Issue (10): 55-62.doi: 10.11707/j.1001-7488.20121010

• 论文 • 上一篇    下一篇

基于SSR标记月季品种鉴定及遗传关系分析

黄平1, 崔鹏娇2, 郑勇奇1, 张川红1, 于雪丹1   

  1. 1. 国家林业局植物新品种分子测定实验室 国家林业局林木培育重点实验室 中国林业科学研究院林业研究所 北京 100091;2. 北京植物园 北京 100093
  • 收稿日期:2012-02-16 修回日期:2012-05-29 出版日期:2012-10-25 发布日期:2012-10-25
  • 通讯作者: 郑勇奇

SSR-Based Molecular Identification and Analysis of Genetic Relationships among Rose Varieties

Huang Ping1, Cui Pengjiao2, Zheng Yongqi1, Zhang Chuanhong1, Yu Xuedan1   

  1. 1. Laboratory of Molecular Testing for New Plant Varieties of State Forestry Administration Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration Institute of Forestry, Chinese Academy of Forestry Beijing 100091;2. Beijing Botanical Garden Beijing 100093
  • Received:2012-02-16 Revised:2012-05-29 Online:2012-10-25 Published:2012-10-25

摘要:

以36个月季品种为材料,应用荧光SSR标记进行月季品种分子鉴定和品种间遗传关系研究。结果显示8个SSR位点检测出85个等位基因变异,等位基因变异范围为6~15个,平均每个位点产生10.6个等位基因变异。结合8个SSR位点,每个品种均具有独特的等位基因表现型,其中位点Ra043a品种鉴定的分辨率最高,产生32个等位基因表现型,区分所有品种最少需要2个SSR位点。品种间成对遗传相似度变幅介于0.105~0.791之间,聚类结果显示36个品种聚为6类; 中国月季聚为一类,与其他月季品种存在遗传差异,起源相近月季品种分别聚为一类。研究认为SSR标记在月季品种鉴定中具备可行性,还可用于品种的遗传关系分析。现代月季遗传背景的深入研究可为未来月季新品种培育、种质资源保存等提供参考依据。

关键词: 月季品种, 简单序列重复, 等位基因表现型, 遗传关系

Abstract:

Fluorescence SSR markers were used to study the feasibility of molecular identification for 36 rose varieties and to investigate the genetic relationships among the varieties. A total 85 allele variations were detected at 8 SSR loci, the number of alleles per locus ranged from 6 to 15 with an average of 10.6 alleles. By integrating the information at 8 loci, unique DNA profiles were obtained for each of the 36 varieties. Ra043a locus had the highest identification, and with it 32 allele phenotypes were detected. At least 2 loci were needed to identify the 36 rose varieties. Pairwise Dice’s genetic similarity varied from 0.105 to 0.791. Cluster analysis showed that the rose varieties were divided into 6 groups approximately. China roses, including those from China and Europe, were clustered into one group, and showed significant genetic variation from the rest varieties. The varieties with similar origin were clustered together. The results indicated that SSR markers used in the study were feasible for variety identification and analysis of genetic relationships among the varieties, providing a profound understanding of the genetic background among modern rose varieties.

Key words: rose variety, simple sequence repeat (SSR), allelic genotype, genetic relationship

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