关键词: 转基因杨树, 抗虫基因, 毒蛋白, 时空表达

Abstract:

We further studied strain PCC1 of transgenic Btcry 3 A poplar 741, which had stable resistance to the pest,and examined the Bt toxic and soluble proteins of twig xylem,leaf and root at different developmental time. The results showed that there was no difference in soluble protein content between transgenic and CK strain,but the soluble proteins of the root and the twig xylem and leaves of different parts in the crown presented a certain regularity. The proteins in twig xylem and roots increased all the time while the proteins in the leaf first decreased and then increased in the whole growing seasons. The expression of Bt toxic protein in xylem increased from upper to lower crown and the expression in leaves decreased from upper to lower crown; the expression of Bt toxic protein in xylem first increased and then decreased with the peak in September. However the expression in leaves and roots did not exhibit a certain regularity with the time on.

Key words: transgenic poplar, insect-resistance gene, Bt toxic protein, temporal and spatial expression