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林业科学 ›› 2010, Vol. 46 ›› Issue (10): 76-82.doi: 10.11707/j.1001-7488.20101012

• 论文 • 上一篇    下一篇

虫害诱导的家榆挥发物对榆紫叶甲寄主选择行为的影响

程彬1,2,付晓霞2,韩启3,张宝民3,张大明2;李兴鹏2,高长启2,孙晓玲4   

  1. 1.东北林业大学林学院 哈尔滨 150040;2.吉林省林业科学研究院 长春 130033;3.吉林省前郭县乌兰图嘎林场 前郭 131121;4.中国农业科学研究院茶叶研究所 杭州 310008
  • 收稿日期:2010-03-14 修回日期:2010-04-29 出版日期:2010-10-25 发布日期:2010-10-25
  • 通讯作者: 孙晓玲

Effects of Herbivore-Induced Ulmus pumila Volatiles on the Host Selection Process of Ambrostoma quadriimpressum

Cheng Bin1,2;Fu Xiaoxia2;Han Qi3;Zhang Baomin3;Zhang Daming2;Li Xingpeng2;Gao Changqi2;Sun Xiaoling4   

  1. 1.College of Forestry Northeast Forestry University Harbin 150040;2.Jilin Provincial Academy of Forestry Sciences Changchun 130033;3.Wulantuga Forest Farm of Qianguo County in Jilin Province Qianguo 131121;4.Tea Research Institute;Chinese Academy of Agricultural Sciences Hangzhou 310008
  • Received:2010-03-14 Revised:2010-04-29 Online:2010-10-25 Published:2010-10-25

摘要:

采用动态顶空采样法结合气相色谱-质谱分析技术(GC-MS)研究受榆紫叶甲成虫危害的家榆挥发物的变化情况,并利用触角电位和“Y”型嗅觉仪比较分析榆紫叶甲雌雄成虫对主要成分的触角电生理和行为反应特点。结果表明: 受榆紫叶甲成虫危害的家榆释放19种成分,其中顺-3-己烯醇、α-蒎烯、柠檬烯、顺-3-乙酸叶醇酯、芳樟醇、水杨酸甲酯、石竹烯、α-法尼烯和(E E)-4 8 12-三甲基-1 3 7 11-十三碳四烯(TMTT) 9种化合物与健康家榆相比有显著差异。除了TMTT,对其余8种化学标准品进行生测的结果表明: 雌性成虫对顺-3-己烯醇、芳樟醇和α-法尼烯有较强的趋性反应,而雄性成虫对α-法尼烯和石竹烯的趋性反应较强。这可为应用引诱剂进行田间监测和控制榆紫叶甲的危害提供理论依据。

关键词: 虫害诱导挥发物, 榆紫叶甲, 质谱分析, 触角电位反应, 行为反应

Abstract:

Herbivore-induced plant volatiles (HIPVs) from Ulmus pumila were collected by a dynamic headspace sampling method, and analyzed by using GC-MS. Furthermore, electroantennogram (EAG) and behavioral responses of Ambrostoma quadriimpressum (Coleoptera: Chrysomelidae)to the main compounds of HIPVs were investigated. Volatile analyses showed that infestations of A. quadriimpressum markedly increased the emission of 9 compounds, α-farnesene, caryophyllene, cis-3-hexenol, α-pinene, limonene, linalool, methyl salicylate, cis-3-hexenyl acetate and TMTT. The results of behavioral bioassays by using 8 synthetic compounds (except for TMTT) revealed that adult females had a stronger response to linalool, α-farnesene and cis-3-hexen-1-ol than to other compounds tested; and adult males had a stronger response to α-farnesene and caryophyllene. EAG results were basically in accordance with Y-tube olfactometer bioassay. These results may provide practical applications for monitoring and controlling A. quadriimpressum populations in the field.

Key words: herbivore-induced plant volatiles, Ambrostoma quadriimpressum, GC-MS, EAG responses, behavioral response