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林业科学 ›› 2010, Vol. 46 ›› Issue (10): 62-68.doi: 10.11707/j.1001-7488.20101010

• 论文 • 上一篇    下一篇

五氯酚胁迫对杨树生长及根际微生物群落的响应特征

周玲莉1,2,姚斌1,仰州3,尚鹤1,韦秀文1,刘方2   

  1. 1.中国林业科学研究院森林生态环境与保护研究所国家林业局森林生态环境重点实验室 北京 100091;2.贵州大学资源与环境工程学院 贵阳 550003;3.中国林业科学研究院热带林业研究所 广州 510520
  • 收稿日期:2009-08-12 修回日期:2009-11-09 出版日期:2010-10-25 发布日期:2010-10-25
  • 通讯作者: 姚斌

Growth and Microbial Community Characteristics in the Rhizosphere of Poplar in Respond to Pentachlorophenol Stress

Zhou Lingli1,2 ;Yao Bin1;Xiang Yangzhou3;Shang He2;Wei Xiuwen1;Liu Fang1   

  1. 1.Key Laboratory of Forest Ecology and Environment;State Forestry Administration Institute of Forest Ecology;Environment and Protection;CAF Beijing 100091;2.College of Resource and Environment Engineering;Guizhou University Guiyang 550003;3.Research Institute of Tropic Forestry;CAF Guangzhou 510520
  • Received:2009-08-12 Revised:2009-11-09 Online:2010-10-25 Published:2010-10-25

摘要:

采用温室盆栽试验方法研究五氯酚污染胁迫对4个杨树品系生长及根际微生物特征的影响。结果表明: 在不同浓度五氯酚污染胁迫下,供试杨树的株高和地径差异显著; 氯仿熏蒸测试结果显示,杨树根际土壤微生物量碳含量随着五氯酚污染浓度增加而下降,表明五氯酚对土壤微生物活性产生毒害作用; Biolog测定结果显示: 五氯酚污染处理的杨树根际土壤微生物平均每孔颜色变化率值始终大于对照,主成分分析结果也显示五氯酚污染处理对杨树根际土壤微生物的碳源利用造成显著影响,初步判断一定浓度范围的五氯酚与根际分泌物的联合作用有利于增强根际土壤微域内特定微生物群落的生理代谢活性,从而增加其碳源利用程度。结果表明: 杨树根际土壤微生物群落的Shannon指数,Simpson指数和McIntosh指数均高于对照,微生物群落的丰富度、均匀度和优势度得到增强,并且五氯酚对土壤微生物的刺激作用随浓度的增加而增强。

关键词: 五氯酚, 杨树, 株高, 地径, 根际微生物, Biolog

Abstract:

The change of growth and microbial community characteristics in the rhizosphere of four types of poplars under pentachlorophenol contamination were investigated with a potting experiment in a green house. The results showed that the height and basal diameter of tested poplars were significant different when treated with different pentachlorophenol concentrations. Concentration of microbial biomass carbon in the rhizosphere soil of poplars, detected by the chloroform fumigation, decreased with increasing pentachlorophenol concentration, which suggested microorganisms were poisoned by high concentration of pentachlorophenol in the soil. Data collected by Biolog measurement demonstrated that the value of average well color development (AWCD) of microorganism in the soil treated with pentachlorophenol was always higher in the rhizosphere of poplars than in the control, and principal component analysis also showed that carbon used by microorganisms in the rhizosphere of poplars was influenced by polluted soil with pentachlorophenol. These results preliminarily implied that the physiological metabolic activity of some specific microbial communities in the rhizosphere soil was enhanced under the integrated effect of a certain range of pentachlorophenol concentration and root exudates in the rhizosphere, which resulted in increasing utilization of carbon source. The indexes of Shannon, Simpson and McIntosh of microbial communities were higher in the rhizosphere soil of poplars than in the control. The richness, evenness and superiority of microbial communities were enhanced, and the stimulated effect of microorganism in response to pentachlorophenol was increased with increasing pentachlorophenol concentration.

Key words: pentachlorophenol, poplar, height, basal diameter, rhizosphere microorganism, Biolog