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林业科学 ›› 2010, Vol. 46 ›› Issue (9): 34-39.doi: 10.11707/j.1001-7488.20100906

• 论文 • 上一篇    下一篇

碳源及植物生长调节剂对雷公藤愈伤组织生长和次生代谢产物含量的影响

李琰1,2,杨广隶1,冯俊涛1,黄正清2,张兴1   

  1. 1.西北农林科技大学无公害农药研究服务中心陕西省生物农药工程技术研究中心 杨凌712100;2.西北农林科技大学生命科学学院 杨凌 712100
  • 收稿日期:2009-05-27 修回日期:2009-07-26 出版日期:2010-09-25 发布日期:2010-09-25
  • 通讯作者: 张兴

Effects of Carbon Sources and Plant Growth Regulators on Callus Growth and Secondary Metabolite Content of Tripterygium wilfordii

Li Yan1,2;Yang Guangli1;Feng Juntao1;Huang Zhengqing2;Zhang Xing1   

  1. 1.Research and Development Center of Biorational Pesticide,Northwest A&F University Shaanxi Province Technology and Engineering Center of Biopesticide Yangling 712100; 2.College of Life Science,Northwest A&F University Yangling 712100
  • Received:2009-05-27 Revised:2009-07-26 Online:2010-09-25 Published:2010-09-25

摘要:

以雷公藤根愈伤组织为材料,添加不同质量浓度的2,4-D,NAA,6-BA,KT或其组合,研究植物生长调节剂及其组合以及碳源对雷公藤愈伤组织的生长、次生代谢产物含量的影响。结果表明: 2,4-D有利于雷公藤愈伤组织的生长和总生物碱的积累,6-BA对愈伤组织的生长起抑制作用,但明显促进愈伤组织中雷公藤内酯醇的形成,KT虽然对愈伤组织的生长起促进作用,但明显抑制愈伤组织中雷公藤内酯醇的形成; 不同质量浓度2,4-D,NAA及与KT,6-BA组合试验表明,1.0 mg·L-1 2,4-D+0.5 mg·L-1 KT明显促进愈伤组织的生长,1.0 mg·L-1 2,4-D+2.0 mg·L-1 KT有利于愈伤组织中总生物碱的形成,而4.0 mg·L-1 NAA+0.5 mg·L-1 6-BA有利于愈伤组织中雷公藤内酯醇的合成。碳源中,对雷公藤愈伤组织的增长量以蔗糖最高,麦芽糖最有利于雷公藤内酯醇的合成,葡萄糖最有利于雷公藤总生物碱的合成。

关键词: 雷公藤, 愈伤组织, 碳源, 植物生长调节剂, 次生代谢产物

Abstract:

The callus of Tripterygium wilfordii root was used as materials, effects of different concentrations of 2,4-D,NAA, KT,or 6-BA and their combinations as well as carbon sources were investigated on callus growth and secondary metabolite contents of T. wilfordii. The results showed that 2,4-D promoted callus growth and accumulation of alkaloids. 6-BA inhibited callus growth, but obviously promoted triptolide accumulation. Although KT was suitable for callus growth,but inhibited significantly triptolide accumulation. The medium supplemented with 1.0 mg·L-12,4-D and 0.5 mg·L-1KT increased the callus growth, while addition of 1.0 mg·L-12,4-D and 2.0 mg·L-1KT increased the alkaloid content.The medium supplemented with 4.0 mg·L-1 NAA and 0.5 mg·L-16-BA increased the triptolide content. Sucrose was best for callus growth, maltose was best for triptolide accumulation, while glucose was best for total alkaloid accumulation.

Key words: Tripterygium wilfordii, callus, carbon sources, plant growth regulators, secondary metabolites