三唑类杀菌剂对苹果褐斑病菌生长发育的毒力及其防病作用

doi:10.11707/j.1001-7488.20090312

林业科学 ›› 2009, Vol. 12 ›› Issue (3): 68-73.doi: 10.11707/j.1001-7488.20090312

三唑类杀菌剂对苹果褐斑病菌生长发育的毒力及其防病作用

赵华¹ 周天仓²程晶晶¹ 李小虎² 黄丽丽¹

（1.西北农林科技大学植物保护学院和陕西省农业分子生物学重点实验室杨凌712100； 2.陕西省蒲城县植保植检站蒲城715500）

收稿日期:2008-01-21 修回日期:1900-01-01 出版日期:2009-03-25 发布日期:2009-03-25

Control Effect of Triazole Fungicides in controlling Marssonina coronaria in Vitro and in Field

Zhao Hua¹,Zhou Tiancang²,Cheng Jingjing¹,Li Xiaohu²,Huang Lili¹

(1.College of Plant protection and Shaanxi Key Lab of Molecular Biology for Agriculture，Northwest A&F University Yangling 712100；2.Pucheng Plant Protection and Quarantine Station Pucheng 715500)

Received:2008-01-21 Revised:1900-01-01 Online:2009-03-25 Published:2009-03-25

摘要/Abstract

摘要：

摘要：戊唑醇和苯醚甲环唑对苹果褐斑病菌生长发育的毒力测定结果表明：对菌丝生长和繁殖体产生具有明显的抑制作用，戊唑醇的EC₅₀分别为0.06和0.055 μg·mL_-1，苯醚甲环唑的EC₅₀分别为0009和0024 μg·mL_-1；但2种药剂对分生孢子萌发的抑制效果较差，其EC₅₀值较菌丝生长和繁殖体产生高10.4～10.5倍，分别为128.825和331.131 μg·mL^-1。苯醚甲环唑对菌丝生长和繁殖体产生的毒力较强，EC₅₀值明显低于戊唑醇，说明其抑制效果较好；而戊唑醇在抑制分生孢子萌发时效果略优于苯醚甲环唑，表现为EC₅₀值相对较小。2年独立的田间试验结果表明： 2种药剂在推荐使用浓度对苹果褐斑病均有较好的防治效果，戊唑醇比苯醚甲环唑的防病效果好，在2005，2006年分别达到82.10%和81.83%，而苯醚甲环唑分别为42.37%和62.38%。新杀菌剂嘧菌酯防病效果达到88.74%与戊唑醇相当，明显优于十二烷基苄基二甲基氯化铵。病害发生初期（陕西关中6月中旬前后）喷施1～2次内吸性杀菌剂即可基本控制当年的病害。建议交替使用这2种药剂及嘧菌酯来控制病害，可以避免病菌产生抗药性。

关键词: 关键词：戊唑醇, 苯醚甲环唑, 苹果褐斑病, 菌丝干重, 分生孢子

Abstract:

Abstract： This study investigated the role of triazole fungicide，tebuconazole and difenoconazole were investigated in controlling the disease in vitro and in field. The fungicides showed a strong inhibition effect on mycelium dry weight，and acervuli formation and a less inhibition effect on conidiagermination by comparing EC₅₀value in vitro EC₅₀values of tebuconazole on mycelium dry weight，acervuli formation and conidia germination were 0.060，0.055 and 128.825 μg·mL^.-1，respectively. EC₅₀ values of difenoconazole were 0.009，0.024 and 331.131 μg·mL^-1，respectively. Difenoconazole showed lower EC₅₀ values than tebuconazole on mycelium growth and acervuli formation on YD and CSA medium，respectively. The fungicides application in an orchard of Pucheng in 2005 and 2006 demonstrated that both fungicides gave high disease control efficacy in application of commended concentration compared to untreated control. Tebuconazole showed higher efficacy than difenoconazole，their control efficiency were 82.10% and 42.37% in 2005，and 81.83% and 62.38% in 2006，respectively. Azoxystrobin，which control efficiency was 88.74%，had no significant difference with tebuconazole. Therefore，alternative use of these fungicides 1～2 times in mid June was recommended to control Marssonina leaf blotch and avoid the resistance of the pathogen to fungicides in this region.

Key words: Key words： tebuconazole, difenoconazole, premature defoliation of apple, mycelium dry weight, conidia germination, disease control

赵华周天仓程晶晶李小虎黄丽丽. 三唑类杀菌剂对苹果褐斑病菌生长发育的毒力及其防病作用[J]. 林业科学, 2009, 12(3): 68-73.

Zhao Hua;Zhou Tiancang;Cheng Jingjing;Li Xiaohu;Huang Lili. Control Effect of Triazole Fungicides in controlling Marssonina coronaria in Vitro and in Field[J]. Scientia Silvae Sinicae, 2009, 12(3): 68-73.

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三唑类杀菌剂对苹果褐斑病菌生长发育的毒力及其防病作用

Control Effect of Triazole Fungicides in controlling Marssonina coronaria in Vitro and in Field

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