关键词: 关键词：落羽杉属, SRAP-PCR, 杂种鉴定

Abstract:

Abstract: In this paper，the authenticity of 4 reciprocal progenies from Taxodium distichum and T. mucronatum was identified by SRAP (Sequence-related Amplified Polymorphism) markers. Firstly, 48 SRAP primer combinations were randomly selected and polymorphism primers between two parents (T. distichum and T. mucronatum ) were selected. As a result, all primers amplifid clear bands, and 29 primers had polymorphism bands of two parents, accounted for 60.4% of all primers, and 14 primer combinations with specific bands both two samples. Authenticity of 4 progenies from T. distichum and T. mucronatum was identified by 12 polymorphism primer combinations selected from above 29 polymorphism primer combinations. The results showed that 4 progenies were all identified to be true hybrids owing to their specific bands from male parent. Among the primer combinations, Me9+Em10 produced specific bands of both male parent and female parent in every progeny, that is to say, with this one primer combination alone, the status of 4 progenies can be identified. Efficiency of molecule identification were much improved. The results of hybrids identification provided a solid evidence for the hybrids further study and application. The experimental results also proved that SRAP molecular marker technology can be effectively applied in the researches on Taxodium.

Key words: Key words: Taxodium, SRAP-PCR, hybrid identification