关键词: 小桐子, 种子, 油体蛋白, 2DPAGE, SDSPAGE

Abstract:

Jatropha curcas，with high oil content seeds，is a promising oil crop for making biodiesel. Oil body is an important constituent of the seeds. In this report，oil body was isolated from the seeds and used to extract proteins. After SDS polyacrylamide gel electrophoresis，eight bands were detected，and two of which (20 ku and 24 ku) likely were isoforms of oleosin. Abundance of the 24 ku was two fold as that of the 20 ku. Two dimensional electrophoresis of the oil body protein extract detected 141 protein spots including 74 acidic proteins and 67 basic proteins. Most of the proteins distributed in the range of pI 5～10 and molecular mass 12～66 ku. Furthermore，the abundant proteins were mainly low molecular mass basic proteins. This was the first report on efficiently isolating oil body and analyzing oil body proteins in the seeds of J. curcas，which laid a foundation for further understanding the function of its oil body.

Key words: Jatropha curcas, seed, oil body proteins, 2D PAGE, SDS PAGE