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林业科学 ›› 2007, Vol. 43 ›› Issue (07): 138-141.doi: 10.11707/j.1001-7488.20070724

• 研究简报 • 上一篇    下一篇

南华松叶蜂病毒DnNPV的发现及其毒力测试

杨苗苗1,2 李孟楼1 曲良建2 王玉珠2 苏琴3 张永安2   

  1. 1.西北农林科技大学林学院,杨凌712100;2.中国林业科学研究院森林生态环境与保护研究所国家林业局森林保护学重点实验室北京100091;3.宁夏隆德县张程乡人民政府,隆德635400
  • 收稿日期:2006-07-25 修回日期:1900-01-01 出版日期:2007-07-25 发布日期:2007-07-25

Finding of Nuclear Polyhedrosis Virus of Diprion nanhuaensis and Its Virulence Test

Yang Miaomiao1,2,Li Menglou1,Qu Liangjian2,Wang Yuzhu2,Su Qin3,Zhang Yong'an2   

  1. 1.College of Forestry,Northwest A&F University Yangling 712100; 2. Institute of Forest Ecology, Environment and Protection, CAF Key Lab. Of Forest Protection of State Forestry Administration Beijing 100091; 3. People's Government of Zhangcheng Town, Longde, Ningxia Longde 635400
  • Received:2006-07-25 Revised:1900-01-01 Online:2007-07-25 Published:2007-07-25

关键词: 南华松叶蜂, 核型多角体病毒, 形态, 祥云新松叶蜂, 南华松叶蜂

Abstract:

One virus of Diprion nanhuaensis was separated from the died D. nanhuaensis, the virus belongs to nuclear polyhedrosis virus, bacilliform virus particle in inclusion body appears SNPV(single nucleocapsid polyhedrosis virus) by electron microscopy. The structure of inclusion body appears polyhedron and close to rotundity, and the length of diameter of inclusion body ranges 0.5~1.0 μm, a little proportion of them shows anomalistic form. There are many bacilliform virus particles in one inclusion body. Six virus particles in one inclusion body take up a large proportion and one virus particle in one inclusion body only takes up a small proportion, the length of virus particle ranges 125~200 nm,and the length of diameter of virus particle ranges 25~30 nm. Meanwhile,the virus can infect the 2~3 instars Neodiprion xiangyunicus and the 2~3 instars D. nanhuaensis in laboratory.

Key words: Diprion nanhuaensis, nuclear poyhedrosis virus, ultrastructure, Neodiprion xiangyunicus, Diprion nanhuaensis