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林业科学 ›› 2005, Vol. 41 ›› Issue (6): 23-27.doi: 10.11707/j.1001-7488.20050604

• 论文及研究报告 • 上一篇    下一篇

酸沉降和铝对马尾松菌根共生体碳代谢影响

谈建康1 孔繁翔2   

  1. 1.南京大学环境学院,南京210093;2.中国科学院南京地理与湖泊研究所,南京210008
  • 收稿日期:2003-09-27 修回日期:1900-01-01 出版日期:2005-11-25 发布日期:2005-11-25

Effects of Acid Precipitation and Al on Carbon Metabolism in Mycorrhizal Symbionts of Pinus massoniana

Tan Jiankang1,Kong Fanxiang2   

  1. 1.Department of Environmental Science and Engineering, Nanjing University Nanjing 210093; 2.Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences Nanjing 210008
  • Received:2003-09-27 Revised:1900-01-01 Online:2005-11-25 Published:2005-11-25

摘要:

对马尾松和马尾松彩色豆马勃菌根植株内碳水化合物及其代谢酶进行测定,研究酸沉降(pH6.0、4.0、3.0、2.0)和铝胁迫(铝理论浓度为75、150μmol·L-1)对其体内糖积累和转运的影响。结果表明:酸沉降和铝胁迫抑制了叶绿素和干质量的积累,两者呈较好的正相关(r=0.949)。叶内可溶性糖含量明显减少;轻度酸沉降促进根、茎内可溶性糖积累,强酸则抑制其积累;酸和铝胁迫抑制了糖向根系转运。低铝胁迫下酸沉降抑制菌根内海藻糖积累,而高铝胁迫促进其积累,并与海藻糖酶活性呈负相关(r=-0.857);轻度酸沉降促进根、茎、叶内葡萄糖积累,强酸沉降抑制其积累;葡萄糖-6-磷酸脱氢酶和海藻糖酶活性受低铝胁迫诱导和高铝胁迫的抑制。菌根的形成能增加叶绿素和干质量的积累,并减少糖向葡萄糖形式转化。

关键词: 酸沉降, 铝, 马尾松, 彩色豆马勃, 碳代谢

Abstract:

This study investigated the carbohydrates and their metabolic enzymes to research the effects of acid precipitation (pH 6.0、4.0、3.0、2.0) and Al (theoretical concentrations were 75, 150 μmol·L-1) on the sugar accumulation and transportation in Pinus massoniana with and without inoculating mycorrhizal fungus Pisolithus tinctorius. The results revealed that the acid precipitation and Al inhibited the chlorophyll and dry weights, between whom the great positive correlation (r=0.949) existed. The contents of soluble sugars decreased in leaves; the light acid precipitation induced sugar accumulation in the roots and stems, but the strong acid precipitation inhibited it; the acid precipitation and Al prevented the sugars transporting down to the roots. The trehalose accumulation was inhibited by the acid precipitation in mycorrhizal roots under the low Al stress, but it was induced under the high Al stress, which showed negative correlation with the activities of trehalase (r=-0.857). The light acid precipitation induced glucose accumulation in the roots, stems and leaves, but the strong acid precipitation inhibited it. The activities of glucose-6-phosphate dehydrogenase and trehalase were induced by the low Al stress, but they were obviously inhibited by the high Al stress. Inoculating mycorrhizal fungus at the roots induced the dry weights, prevented the sugars transforming into glucoses.

Key words: acid precipitation, Al, Pinus massoniana, Pisolithus tinctorius, carbon metabolism