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林业科学 ›› 2002, Vol. 38 ›› Issue (1): 29-35.doi: 10.11707/j.1001-7488.20020105

• 论文及研究报告 • 上一篇    下一篇

不同种泡桐叶片愈伤组织诱导及其植株再生

范国强1 翟晓巧2 蒋建平4 刘心诚3   

  1. 河南农业大学泡桐研究所,郑州450002;许昌林业技术推广站,许昌461000
  • 收稿日期:2001-10-24 修回日期:1900-01-01 出版日期:2002-01-25 发布日期:2002-01-25

CALLUS INDUCTION FROM PAULOWNIA PLANT LEAVES AND THEIR PLANTLET REGENERATIONS

Fan Guoqiang,Zhai Xiaoqiao,Jiang Jianping,Liu Xincheng   

  1. Institute of Paulownia, Henan Agricultural University Zhengzhou450002;Xuchang Forestry Popularization Station,He'nan Province Xuchang461000
  • Received:2001-10-24 Revised:1900-01-01 Online:2002-01-25 Published:2002-01-25

摘要:

在确定5种泡桐叶片诱导愈伤组织基本培养基为MS的基础上,筛选出了毛泡桐(Paulownia tomentosa)、南方泡桐(Paulownia australis)、白花泡桐(Paulownia fortunei)、兰考泡桐(Paulownia elongata)和豫杂一号泡桐(P.tomentosa×P.fortunei)叶片愈伤组织诱导的最适培养基(分别为MS+0.5NAA +4BA、MS+0.3NAA+2BA、MS+0.5NAA+4BA、MS+0.3NAA+6BA和MS+0.3NAA+8BA) ,然后,从18个不同浓度NAA和BA组合的MS培养基中,找出了它们叶片愈伤组织诱导芽分化最适培养基(分别为MS+0.3NAA+12BA、MS+0.3NAA+12BA、MS+0.5NAA+12BA、MS+0.5NAA+12BA和MS+0.7NAA+12BA) ,最后,找出了5种泡桐芽诱导根的最适培养基(分别为1/2MS+0.1NAA、1/2MS +0.1NAA、1/2MS、1/2MS +0.3NAA和1/2MS +0.5NAA)。这些结果为开展泡桐基因工程研究和利用不同种泡桐叶片原生质体融合培育泡桐新品种提供了参考。

关键词: 泡桐, 愈伤组织诱导, 再生植株, 培养基, 激素

Abstract:

This paper dealt with callus induction from different Paulownia plant leaves and their plantlet regenerations. The results indicated that MS meidium was optimum one for induction of callus from 5 species of Paulownia plant leaves; that induction media from plant leaves of Paulownia tomentosa, P. australis, P. fortunei, P. elongata and P. tmentosa×P. fortunei were MS+0.5NAA+4BA、MS+0.3NAA+2BA、MS+0.5NAA+4BA、MS+0.3NAA+6BA and MS+0.3NAA+8BA; that bud induction ones from those plant leaf calli were MS+0.3NAA+12BA, MS+0.3NAA+12BA, MS+0.5NAA+12BA, MS+0.5NAA+12BA, MS+0.7NAA+12BA;and that 1/2MS+0.1NAA, 1/2MS+0.1NAA, 1/2MS, 1/2MS+0.3NAA and 1/2MS+0.5NAA were those rooting media.

Key words: Paulownia, Callus induction, Plantlet regeneration, Medium, Hormone