绿僵菌侵染光肩星天牛幼虫过程的透射电镜观察

doi:10.11707/j.1001-7488.20100518

林业科学 ›› 2010, Vol. 46 ›› Issue (5): 113-115.doi: 10.11707/j.1001-7488.20100518

绿僵菌侵染光肩星天牛幼虫过程的透射电镜观察

王达^1,2,袁芳芳³,黄大庄²,刘春延⁴,毕华明⁴

1.上海市农业科学院花卉研究中心上海201106；2.河北农业大学林学院保定071000；3.廊坊市林业局廊坊065000；4.塞罕坝机械林场围场县068400

收稿日期:2008-09-26 修回日期:1900-01-01 出版日期:2010-05-25 发布日期:2010-05-25
通讯作者: 黄大庄

Infection Process of Metarhizium anisopliae in Anoplophora glabripennis Larvae Observed with Transmission Electron Microscopy

Wang Da^1,2;Yuan Fangfang³;Huang Dazhuang²;Liu Chunyan⁴;Bi Huaming⁴

1.Flower Research Center，Shanghai Academy of Agricultural SciencesShanghai 201106；2.Forestry College，Hebei Agricultural UniversityBaoding 071000；3.Langfang Forestry AdministrationLangfang 065000;4.Saihanba Machinery Tree FarmWeichang 068400

Received:2008-09-26 Revised:1900-01-01 Online:2010-05-25 Published:2010-05-25

摘要/Abstract

摘要：

应用透射电镜观察并研究光肩星天牛幼虫感染绿僵菌后体壁和中肠发生的病理变化。结果表明：接菌后16 h，寄主表皮层中观察到少量菌丝段，至24 h菌丝已普遍侵入，芽管在穿透过程中表现了机械压力和酶活性。36～48 h菌丝即可穿透体壁进入血腔。菌丝和菌丝段经血淋巴循环侵入各器官组织。48～72 h，随着菌丝和菌丝段在肠壁细胞中迅速大量增殖，肠壁组织逐渐被破坏，中肠微绒毛脱落，肠壁细胞形成空泡，围食膜被分解消失，最终中肠组织崩溃、解体。

关键词: 绿僵菌光, 肩星天牛, 侵染过程, 病理变化, 透射电镜

Abstract:

The pathological changes in integument and midgut of Anoplophora glabripennis larvae infected by Metarhizium anisopliae were studied with a transmission electron microscopy.The results indicated that: in 16 h post-inoculation,the host cuticle was invaded by a few hyphal bodies.Up to 24 h hyphae widely penetrated into host cuticle.The germ tubes showed mechanical pressure and enzymatic activity during the penetration.The hyphae penetrated through the integument and entered the haemocoele from 36 h to 48 h.The hyphae and hyphal bodies invaded all organs and tissues via hemolymph circulation.With the quick and mass reproduction of the hyphae and hyphal bodies in the midgut epithelium,the midgut tissues were gradually destroyed,the midgut microvilli exfoliated,vacuole formed in the midgut epithelium,the peritrophic membrane was decomposed and disappeared,eventually the midgut tissues collapsed and disintegrated from 48 h to 72 h.

Key words: Metarhizium anisopliae, Anoplophora glabripennis, infection process, pathological changes, transmission electron microscopy

王达;袁芳芳;黄大庄;刘春延;毕华明. 绿僵菌侵染光肩星天牛幼虫过程的透射电镜观察[J]. 林业科学, 2010, 46(5): 113-115.

Wang Da;Yuan Fangfang;Huang Dazhuang;Liu Chunyan;Bi Huaming. Infection Process of Metarhizium anisopliae in Anoplophora glabripennis Larvae Observed with Transmission Electron Microscopy[J]. Scientia Silvae Sinicae, 2010, 46(5): 113-115.

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绿僵菌侵染光肩星天牛幼虫过程的透射电镜观察

Infection Process of Metarhizium anisopliae in Anoplophora glabripennis Larvae Observed with Transmission Electron Microscopy

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