苏云金杆菌杀虫晶体蛋白基因转化美洲黑杨的研究

Abstract

Abstract:

The effective regenetation system for Populus deltoides was developed firstly in the experiment. Based on the system, Agrobacterium tumefaciens LBA 4404 strains hauboring the Bt. toxin gene expression vectors pB48.214 and pB48.215 respectively were uesd for transformation of poplar plants by means of the leaf disc method. After transformating, adventitious buds were formed at the cut of explant on the media with two concentrations of kanamycin 50mg/l and 30mg/l nearly one month later. Variance analysis showed that the effects of two kanamycin concentrations on the transformation rate and mean adventitious bud numbers were significant at p < 0.05. When shoots reached 1cm long, they were transferred to rooting media and supplemented with 30mg/l and 50mg/l kanamycin and the rooting percentage was 9.62% and 8.02% respectively. PCR analysis showed that Bt. genes were successfully transformed into Populus deltoides chromosomes.

Key words: Bt. gene, Populus deltoides, Transformation, PCR analysis

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STUDY ON THE PLANT REGENERATION FROM POPULUS DELTOIDES EXPLANT TRANSFORMED WITH BT. TOXIN GENE

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