Scientia Silvae Sinicae ›› 2022, Vol. 58 ›› Issue (1): 52-61.doi: 10.11707/j.1001-7488.20220106
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Weixi Zhang1,Yanbo Wang1,2,Changjun Ding1,Wenxu Zhu1,3,Xiaohua Su1,*
Received:
2021-01-13
Online:
2022-01-25
Published:
2022-03-08
Contact:
Xiaohua Su
CLC Number:
Weixi Zhang,Yanbo Wang,Changjun Ding,Wenxu Zhu,Xiaohua Su. Detection of Horizontal Transfer of the Exogenous Gene in Adult Trees of Transgenic Populus alba × P. berolinensis in a Field Trial and Successive Years of Monitoring of Soil Microorganism[J]. Scientia Silvae Sinicae, 2022, 58(1): 52-61.
Fig.1
PCR detection of exogenous gene JERF 36 A: PCR detection of exogenous gene JERF 36 (1: Non-transgenic control 9#; 2: Understory weed; 3: Total DNA in soil; 4: Seedling leaves of roadside poplar beside the test field; 5: Kanamycin-resistant strain 1; 6: Kanamycin-resistant strain 2; 7: Transgenic poplar ABJ01; CK+: Plasmid positive control; CK-: Deionized water negative control); B: PCR detection of 16S rRNA(M: DNA marker; H: Deionized water negative control; 1: Total DNA in soil 1; 2: Total DNA in soil 2; 3: Kanamycin-resistant strain 1; 4: Kanamycin-resistant strain 2)."
Fig.2
Total number of microorganisms of 9-11 year-old(2015-2017) poplar test field and the temperature and raining days in different months 9#: Non-transgenic plants; ABJ01: Transgenic plants. The same below. Different lowercase letters indicate significant differences in the number of microorganisms in different months at the 0.05 level; "*" means significant differences in the number of microorganisms in different years at the 0.05 level."
Fig.3
Total numbers of bacteria, actinomycetes, and fungi in soil of 9-11 years old (2015—2017) poplar test field A: Total number of soil microorganisms in 9-year-old poplar plantation from May to August; B: Total number of soil microorganisms in 10-year-old poplar plantation from May to October; C: Total number of soil microorganisms in 11-year-old poplar plantation from May to October. Different lowercase letters indicate significant differences in the number of microorganisms in different months at the 0.05 level."
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