Scientia Silvae Sinicae ›› 2021, Vol. 57 ›› Issue (8): 195-204.doi: 10.11707/j.1001-7488.20210820
• Scientific notes • Previous Articles
Yunxin Cen1,Jia Liu1,Faju Chen1,Jingyuan Yang2,Qiang Liu2,Tao Wang2,Hongwei Liang1,*
Received:
2020-10-16
Online:
2021-08-25
Published:
2021-09-30
Contact:
Hongwei Liang
CLC Number:
Yunxin Cen,Jia Liu,Faju Chen,Jingyuan Yang,Qiang Liu,Tao Wang,Hongwei Liang. Agrobacterium-Mediated Genetic Transformation System of Catalpa bungei[J]. Scientia Silvae Sinicae, 2021, 57(8): 195-204.
Table 1
GUS transient expression rate of Agrobacterium-mediated orthogonal test"
编号 No. | 预培养时间 Pre-culture duration/d | 菌株 Strain | 菌液浓度 Bacteria concentration (OD600) | AS浓度 AS concentration/(μmol·L-1) | 侵染时间 Infection duration/min | 共培养时间 Co-culture duration/d | GUS瞬时表达率 GUS transient expression rate(%) |
1 | 1 | EHA105 | 0.5 | 100 | 10 | 3 | 43.57 |
2 | 1 | GV3101 | 0.7 | 200 | 20 | 4 | 59.00 |
3 | 1 | LBA4404 | 0.9 | 300 | 30 | 5 | 47.57 |
4 | 2 | EHA105 | 0.5 | 200 | 20 | 5 | 53.94 |
5 | 2 | GV3101 | 0.7 | 300 | 30 | 3 | 61.08 |
6 | 2 | LBA4404 | 0.9 | 100 | 10 | 4 | 53.98 |
7 | 3 | EHA105 | 0.7 | 100 | 30 | 4 | 35.46 |
8 | 3 | GV3101 | 0.9 | 200 | 10 | 5 | 48.41 |
9 | 3 | LBA4404 | 0.5 | 300 | 20 | 3 | 50.09 |
10 | 1 | EHA105 | 0.9 | 300 | 20 | 4 | 55.01 |
11 | 1 | GV3101 | 0.5 | 100 | 30 | 5 | 48.33 |
12 | 1 | LBA4404 | 0.7 | 200 | 10 | 3 | 46.38 |
13 | 2 | EHA105 | 0.7 | 300 | 10 | 5 | 68.41 |
14 | 2 | GV3101 | 0.9 | 100 | 20 | 3 | 45.54 |
15 | 2 | LBA4404 | 0.5 | 200 | 30 | 4 | 50.00 |
16 | 3 | EHA105 | 0.9 | 200 | 30 | 3 | 46.11 |
17 | 3 | GV3101 | 0.5 | 300 | 10 | 4 | 60.53 |
18 | 3 | LBA4404 | 0.7 | 100 | 20 | 5 | 51.04 |
Table 2
Visual analysis of GUS transient expression rate in orthogonal test"
因素和 Sum of factors | GUS瞬时表达率GUS transient expression rate | |||||
预培养时间 Pre-culture duration | 菌株 Strain | 菌液浓度 Bacteria concentration | AS浓度 AS concentration | 侵染时间 Infection duration | 共培养时间 Co-culture duration | |
K1(%) | 2.999 | 3.025 | 3.065 | 2.779 | 3.213 | 2.928 |
K2(%) | 3.329 | 3.229 | 3.214 | 3.038 | 3.146 | 3.140 |
K3(%) | 2.916 | 2.991 | 2.966 | 3.427 | 2.885 | 3.177 |
R(%) | 0.413 | 0.238 | 0.247 | 0.648 | 0.327 | 0.249 |
Fig.8
CTK and IAA concentrations and CTK/IAA ratio in different embryogenic callus A: Embryogenic callus of C. bungei grows normally on the subculture medium; B: Embryogenic callus of C. bungei on the subculture medium added with 60 mg ·L-1 Kana; C: Resistant embryogenic callus on screening medium after Agrobacterium infection. The same below."
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