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Scientia Silvae Sinicae ›› 2015, Vol. 51 ›› Issue (9): 96-105.doi: 10.11707/j.1001-7488.20150913

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Effect of Purification on Antioxidative Activity of Lignan Fractions from Schisandra chinensis

Gu Yanfei1, Yan Boqian2, Ding Ke1, Wang Zongyi1, Zhang Zhongjie3, Han Tao1   

  1. 1. Beijing Key Laboratory of Agricultural Product Detection and Control of Spoilage Organisms and Pesticide Residue College of Food Science and Engineering, Beijing University of Agriculture Beijing 102206;
    2. College of Plant Science and Technology, Beijing University of Agriculture Beijing 102206;
    3. Academy of State Administration of Grain Beijing 100037
  • Received:2014-09-15 Revised:2014-10-14 Online:2015-09-25 Published:2015-10-16

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Abstract:

[Objective] Schisandra chinensis is the dried ripe fruit of Schisandra chinensis. Lignans are the major active ingredients of S. chinensis, which have many pharmacological actions. So far in China, except for the raw of S.chinensis, existing products are mostly Chinese patent drugs. Studies on antioxidant activities under different purities will provide theoretical references for developing lignan-rich Schisandra functional foods. [Method] Microwave-ultrasound extraction methods were used in extracting lignans from S.chinensis, and purify the crude extracts of lignan from S.chinensis. It is preliminarily purified with AB-8 macroporous resin and then eluted with pH10.0, 100% ethanol. This step can remove macromolecular substances, playing a role in preliminarily purifying the crude extracts of lignan from S.chinensis.Qualitive analyses were made by high performance liquid chromatography (HPLC), the separated conditions of Venusil XBP C18 (150 mm×4.6 mm,5 μm), methanol(B)-water(A) was used as gradient mobile phase (0-7 min 67%B, 7-13 min 67%-75%B, 13-22 min 75%-80%B, 22-30 min 80%-67%B),the detection wavelength was set at 220 nm,the flow rate was 0.8 mL·min-1, sample solution was 10 μL, the column temperature was as room temperature. And the purity of lignans was calculated by area normalization: in the crude extracts, the purity of lignans was 29.33% and the purity of lignans preliminarily purified by macroporous resin was 58.95%. Then the crude extracts were further purified by preparative liquid chromatography (PLC), the separated conditions of Adsorbosphere XL C18 (250 mm ×22 mm,5 μm), methanol(A)-water(B) was used as gradient mobile phase (0-10 min 75%A, 10-15 min 75%-78%A, 15-38 min 75%-80%A),the detection wavelength was set at 220 nm,the flow rate was 10 mL·min-1, sample solution was 3 mL, the column temperature was as room temperature.Four lignan monomers were obtained by PLC; purities of schisandrin, schisantherin A, deoxyschizandrin and schisandrin B were 95.40%, 98.85%, 94.76% and 96.44%, respectively. The reducing capacities and free radical scavenging capacities (·O2-,·OH, H2O2, DPPH and ABTS) of lignans from S.chinensis of different purities were compared, and an evaluation was made for effect of purification on antioxidant activities of lignans.[Result] In the range of 0.1 to 0.5 mg·mL-1, purified lignan extracts showed weaker antioxidant activity than that of unpurified crude lignan extracts; in the monomers obtained by PLC, schisandrin B showed a stronger antioxidant activity.[Conclusion] The crude extracts of lignan from S.chinensis contains polysaccharides and other ingredients, polysaccharide has antioxidant capacity. The synergistic reactions among lignans, polysaccharides and other ingredients make a stronger antioxidant capacity.Yet after purifying the crude extracts with AB-8 macroporous resin, the polysaccharides was removed, the antioxidant capacity of individual lignan is relatively weaker. These results indicate that as the degree of purification is increased, the single degree of material's component is higher, but biological activity of the substance is not necessarily increased as well. And in the monomers obtained by PLC, schisandrin B showed a stronger antioxidant activity, the reason may be related to the substituent on its biphenyl ring and octylene ring.

Key words: Schisandra chinensis, lignan, antioxidation, purification, PLC

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