桑椹发育中花青素、叶绿素含量变化及相关基因的表达分析

doi:10.11707/j.1001-7488.20140908

Abstract

Abstract:

The contents of anthocyanins and chlorophyll of fruits of Morus alba ‘Changjiang 1’ at the different developmental stages were measured, and the expression levels of anthocyanin biosynthesis related genes, the encoding genes of Rubisco and pheophorbide a oxygenase (PaO) were analyzed. The concentration of anthocyanins increased gradually, while the content of chlorophyll first significantly reduced and then increased during the fruit development process. The anthocyanin related genes showed different patterns, the expression levels of RBCL and RBCS were gradually decreasing, and PaO1 and PaO2 showed a reduction in the expression levels at early stage and increase in the following stage, and then the coding mRNA disappeared at stage S5. ABA, ethephon and 1-MCP were used to treat the mulberry fruit. ABA and ethephon induced the biosynthesis of anthocyanins and chlorophyll, improved the expression levels of anthocyanin related genes and downregulated the expression of Rubisco encoded genes. The 1-MCP can inhibited the biosynthesis of anthocyanins by downregulating the expression levels of anthocyanin related genes.

Key words: mulberry fruit, anthocyanin, chlorophyll, ethylene, ABA, 1-MCP

CLC Number:

S718.43

Liu Changying, Li Jun, Zhao Aichun, Wang Xiling, Lü Ruihua, Wang Xiaohong, Lu Cheng, Yu Maode. Changes of Anthocyanin and Chlorophyll Content, and Expression Levels of Related Genes during Development Process of Mulberry Fruit[J]. Scientia Silvae Sinicae, 2014, 50(9): 59-66.

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Changes of Anthocyanin and Chlorophyll Content, and Expression Levels of Related Genes during Development Process of Mulberry Fruit

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