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Scientia Silvae Sinicae ›› 2014, Vol. 50 ›› Issue (3): 45-54.doi: 10.11707/j.1001-7488.20140307

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Influences of Growth Media, and Hormone Types and Concentrations on Cutting Propagation of Rhododendron latoucheae

Zhang Lehua, Wang Shusheng, Shan Wen, Li Xiaohua, Wang Kaihong, Wang Zhaohong   

  1. Lushan Botanical Garden, Jiangxi Province and Chinese Academy of Sciences Lushan 332900
  • Received:2013-07-25 Revised:2013-12-22 Online:2014-03-25 Published:2014-04-16

Abstract:

Cuttings of annual woody branches of Rhododendron latoucheae were treated with different hormone types and concentrations in different growing media and the influences of the three factors and their interaction on the cutting propagation were studied using factorial design. The propagation effect of each factor combination was comprehensively evaluated by the subordinate function method. The results showed that, the hormone types had the greatest influence on cutting propagation among the three main factors, followed by hormone concentrations, and the two factors had significant influences on the most cutting propagation parameters. Media had the least influence on cutting propagation, and it nevertheless had significant influence on callusing percentage, rooting percentage and above-ground growth parameter. The analysis results of the interaction of the three factors revealed that, the interaction of hormone types and the concentrations had the biggest effects on cutting propagation, and it brought about most significant difference on all parameters except the number and the length of new shoot. The interaction of media and hormone types had significant influence on rooting parameters. The interaction of media and hormone concentration was least, with significant influence only on callusing percentage and new shoot percentage. The interaction of media, hormone types and concentrations also had significant influences on several rooting parameters. The results of comprehensive evaluation using the subordinate function method showed that the combination of soil+sand (3:1) medium and GA3(50 mg·L-1) had the biggest value of subordinate function, and it led to the highest rooting percentage (78.33%) and retention percentage of old leaves (90.00%), and the lowest rotting percentage (8.33%). The other propagation parameters also well performed with the combination in comparison with all other combinations. Thus, this combination should be best for cutting propagation of Rh. latoucheae.

Key words: Rhododendron latoucheae, cutting propagation, media, hormones, propagation parameters

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