根际促生细菌(PGPR)对冬枣根际土壤微生物数量及细菌多样性影响

doi:10.11707/j.1001-7488.20130811

Abstract

Abstract:

A dominant micro-organism strain was selected from rhizosphere soil of six-year-old Ziziphus jujuba trees by biological assay. A plant growth-promoting rhizobacteria (PGPR) fertilizer (PF) was made by adsorbing the PGPR to decomposed chicken manure (DC). The traditional chloroform fumigation method and modern Terminal Restriction Fragment Length Polymorphism (T-RFLP) method were used to evaluate the effect of PF, DC and ordinary biological fertilizer (NF) on the microbial populations, the bacterial diversity and the dynamic soil microbial carbon in the Z. jujuba rhizosphere soil. Results showed that, compared with NF treatment, PF increased significantly the bacterial quantity and the total microorganism populations in the rhizosphere soil, but decreased significantly the fungus populations. However PF had no significant effect on actinomycetes populations in the rhizosphere soil. There was a significant difference in T-RFLP profiles of different treatments. PF had the highest Margalef index, Shannon index, and Pielou index among all the four treatments. Principal component analysis on terminal restriction fragments (T-RFs) of the different treatments showed that an independent group of bacterial community structure was formed in PF, and the other independent group was formed in NF, DC and CK treatments, respectively. Additionally, during the whole growth period, PF could supply the stable soil microbial carbon in compared with other treatments. Consequently, application of PGPR fertilizer was beneficial on the ecological environment of Z. jujuba rhizosphere soil, with the higher cultural microorganism populations and soil microbial carbon, and abundant bacterial diversity.

Key words: plant growth-promoting rhizobacteria(PGPR), chicken manure, Ziziphus jujuba, rhizosphere, bacterial diversity, microbial carbon

CLC Number:

Liu Fangchun, Xing Shangjun, Ma Hailin, Ding Yanqin, Chen Bo, Du Binghai. Effect of Plant Growth-Promoting Rhizobacteria (PGPR) on the Microorganism Population and Bacterial Diversity in Ziziphus jujuba Rhizosphere Soil[J]. Scientia Silvae Sinicae, 2013, 49(8): 75-80.

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Effect of Plant Growth-Promoting Rhizobacteria (PGPR) on the Microorganism Population and Bacterial Diversity in Ziziphus jujuba Rhizosphere Soil

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