Abstract:

In order to screen culture conditions of a bioreactor for multiplying blueberry (Vaccinium uliginosum), the raft culture method was used in an air-lift bioreactor to study effects of inoculation density, air volume, and light intensity on proliferation and growth of shoots. The results indicated that a mass of shoots were able to be produced by using raft culture in a bioreactor. Inoculation density affected multiplication of blueberry, 60 inoculates in a 5 L bioreactor were the most suitable inoculation density, which led 38.6 adventitious shoots per explant, and total 2 316 vigorous shoots per bioreactor were obtained. Air volume rate of 100 mL·min^-1 had the best effect on proliferation, and it produced significantly higher growth of shoots, more shoot numbers, greater fresh and dry mass than did the air volume groups of 50 and 150 mL·min^-1. Multiplication of shoots was also affected by light intensity, and 45 μmol·m^-2s^-1 was better than either 30 or 60 μmol·m^-2s^-1 for producing dark green and vigorous shoots in the bioreactor.

Key words: blueberry(Vaccinium uliginosum), tissue culture, bioreactor, inoculation density, air volume, light intensity