Abstract:

The vegetative storage proteins(VSPs) in branches of Ginkgo biloba were isolated and identified by using light- and electron-microscope, SDS-PAGE, immunoenzyme optical-microscopy localization and immunogold electron-microscopy localization. The cytological characteristic and dynamic changes of VSPs were preliminarily studied. The results were as follows: The antigens of vegetative storage protein of 32 and 36 ku were separated and prepared by using SDS-polyacrylamide gel electrophoresis, antibodies of vegetative storage proteins of 32 and 36 ku were successfully achieved with immuniting New Zealand rabbits. VSPs were found mainly in the secondary phloem parenchyma cells of one-year-old twigs during deciduous stage and they were accumulated in vacuole in different forms such as nubbly, granular, and homogeneous under electron microscope. The protein inclusions of branches almost disappeared during new shoot development, with obviously seasonal change.

Key words: Ginkgo biloba, vegetative storage protein(VSPs), isolation and identification, immuno-labeling analyzing