Abstract:

MhGLR gene was identified from Malus hupehensis var. pingyiensisby RACE on the basis of apple expressed sequence tag (EST) database． MhGLR cDNA was 3 600 bp in length and encoded a protein molecule with 946 amino acids, whose molecular mass was estimated of 107.09 ku． Sequence alignment of MhGLR with other members of the GLR (glutamate receptor) family showed that MhGLR was closely related to clade Ⅲ Arabidopsis GLRs and was closest to AtGLR36. Therefore, we named it MhGLR(GenBank accession No.(EF432572)． Hydropathy analysis indicated that MhGLR36 contained six signature domains of animal ionotropic GluRs． Quantitative realtime PCR analysis demonstrated that MhGLR as expressed in roots，stems and leaves．The expression level in leaves was higher than that in roots and stems． Lglutamate and IBA treatments were ableto induce the expression of MhGLR36 in roots． To study the function of [MhGLR we introduced the antisense MhGLRunder the control of 35S promoter of Cauliflower mosaic virus into Malus domestica cv. Royal Gala plants．

Key words: Malus hupehensis var. pingyiensis, [MhGLR ene, gene cloning, expression, transformation