休眠期珙桐种子cDNA文库构建及EST分析*

doi:10.11707/j.1001-7488.20091012

Scientia Silvae Sinicae ›› 2009, Vol. 12 ›› Issue (10): 69-73.doi: 10.11707/j.1001-7488.20091012

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Construction of cDNA Library and Analysis of the Expressed Sequenced Tags (ESTs) Characteristics of Dormant Seeds of Davidia involucrata

Qi Gang^1,2,Su Zhixian²,Li Jingtao³,Ruan Qiping³

1.School of Life Sciences, China West Normal University Nanchong 637002； 2.Key Laboratory of Ecology and Environmental Protection Technology of Sichuan Province Mianyang 621000；3.Key Laboratory for Molecular Biology and Biopharmaceuticals of Mianyang Normal University Mianyang 621000

Received:2008-08-06 Revised:1900-01-01 Online:2009-10-25 Published:2009-10-25
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Abstract

Abstract:

A cDNA library was constructed with dormant Davidia involucrata seeds by using SMART cDNA Library Construction Kit. The library had a titer of 1.5×10⁶ pfu·mL^-1， with recombinant rate of 98%. The inserted cDNA fragments ranged mainly from 0.5 to 1.0 kb. Total 148 ESTs were obtained from randomly sequencing, and 127 uniESTs were obtained by contiganalsis. By searching on line with BlastN and BlastX, 75 uniESTs were determined to be the tags of genes with putative function and high similarity, 32 uniESTs had low similarity and 13 uniEST had no significant similarity to any protein or DNA sequence in the databases. The present ESTs provide a valuable resource for further research on similarity protein function and molecule mechanism of Davidia involucrata seeds.

Key words: Davidia involucrata, seed, dormancy, cDNA library, ESTs

CLC Number:

Qi Gang;Su Zhixian;Li Jingtao;Ruan Qiping. Construction of cDNA Library and Analysis of the Expressed Sequenced Tags (ESTs) Characteristics of Dormant Seeds of Davidia involucrata[J]. Scientia Silvae Sinicae, 2009, 12(10): 69-73.

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Construction of cDNA Library and Analysis of the Expressed Sequenced Tags (ESTs) Characteristics of Dormant Seeds of Davidia involucrata

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