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Scientia Silvae Sinicae ›› 2006, Vol. 42 ›› Issue (6): 141-144.doi: 10.11707/j.1001-7488.20060623

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ITS Analysis of Quarantine Insect Opogona sacchari (Lepidoptera: Hieroxestidae) from Different Hosts

Shen Jie1,Gao Qikang2,Shen Youlian3,Lou Binggan2   

  1. 1. Forest Pest Control and Quarantine Station of Zhejiang Province Hangzhou 310004; 2. Zhejiang University Hangzhou 310029;3. Cixi Forest Pest Control Station of Zhejiang Cixi 315300
  • Received:2005-05-09 Revised:1900-01-01 Online:2006-06-25 Published:2006-06-25

Abstract:

The internal transcribed spacer (ITS) of Opogona sacchari, a important quaratine pest, from different hosts was studied by using the special primers designed by the highly conserved region sequence of 18S and 28S rDNA region, and the ITS sequences of Opogona sacchari from diffirent hosts were compared. The total length of O. sacchari ITS1, ITS2 and 5.8S rDNA is 987 bp. The average of G+C contents is 47.2%. Length of ITS1 and ITS2 fragment is 443 bp and 358 bp. The ITS1 and ITS2 from different hosts are identical or unsignificantly difference.

Key words: Opogona sacchari, internal transcribed spacer, host, quarantine