真菌诱导子促进桑黄黄酮类物质积累及主要作用机制

doi:10.11707/j.1001-7488.LYKX20250281

Abstract

Abstract:

Objective: This study aims to explore the effects of fungal elicitors from different fungi and different types of fungal elicitors from the same fungus on the growth of mycelia & fruiting bodies and flavonoid content, effective medicinal components of Sanghuangporus vaninii, in order to comprehensively evaluate the efficiency of fungal elicitors in different growth stages and culture environments. Method: Three different types of fungal elicitors were prepared from 11 different soil fungi. A total of 33 different fungal elicitors were used to treat the mycelia of S. vaninii. The biomass, flavonoid content and flavonoid production were statistically analyzed, and three optimal fungal elicitors were screened out based on the three indicators. The real-time quantitative PCR was used to preliminarily verify the molecular mechanism by which three optimal fungal elicitors enhanced flavonoid content. The induction conditions of fungal elicitors were optimized by the response surface method. Three optimal fungal elicitors were applied to the fruiting bodies to verify the accumulation of flavonoid content. Finally, non-targeted metabolomics analysis was conducted on the mycelia and the fermentation broth of strain HSL, which had a better effect in increasing the flavonoid content of S. vaninii. Result: Three optimal fungal elicitors (NL-19M, NL-19ME and HSLFE) had the best induction effects on the biomass, flavonoid content and flavonoid production of S. vaninii. Moreover, the three fungal elicitors promoted the accumulation of flavonoid content by increasing the expression levels of different key enzymes in the flavonoid biosynthesis pathway. There were differences in the optimization conditions for the biomass, flavonoid content and flavonoid production by the elicitors of the same fungus. The flavonoid contents of the fruiting bodies treated with NL-19M, NL-19ME and HSLFE were all higher than those of the control. The active ingredients of fungal elicitors may be lipids and lipid-like molecules. Conclusion: Different optimal fungal elicitors treatments can activate the expression of different key enzymes for flavonoid biosynthesis and have different induction conditions, reflecting the complexity of fungal metabolic regulation and revealing the regulatory mechanism of fungal elicitors on the flavonoid biosynthesis. This study provides a more complete and systematic theoretical basis for the commercial application of fungal elicitors and the establishment of a technical system for improving the quality and efficiency of under-forest economy including medicinal and edible fungi.

Key words: fungal elicitors, Sanghuangporus vaninii biomass, flavonoid content, optimized conditions, fruiting body

CLC Number:

Q939.96

Yuwei Zhang,Mengting Mo,Bo Shu,Jiajia Yao,Zhilin Yuan. Fungal Elicitors Promote Flavonoid Biosynthesis in Sanghuangporus vaninii and the Underlying Mechanisms[J]. Scientia Silvae Sinicae, 2026, 62(3): 146-160.

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菌株编号 Isolate No.	菌种 Species	菌株来源 Strain origin	参考文献 Reference
QYL-10	Clitopilus hobsonii	琴叶栎根系Quercus lyrata root	靳微，2019
NL-19	C. hobsonii	牛栎根系Q. michauxii root	靳微，2019
XST	C. hobsonii	黄金小神童根系Cymbidium golden root	姚娜等，2024
HSL	Clitopolius sp.	黄山栎根系Q. stewardii root	靳微，2019
Cab	C. abprunulus	子实体Fruiting body	Jian et al.， 2020
84496	C. prunulus	子实体Fruiting body	CFCC
50334	C. scyphoides	子实体Fruiting body	CFCC
HBPOP-BS-1	Mortierella alpina	毛白杨根际土壤Populus tomentosa rhizosphere soil	潘雪玉，2018
HBPOP-BS-2	M. alpina	毛白杨根际土壤P. tomentosa rhizosphere soil	潘雪玉，2018
SML-TX-18	Atractiella rhizophila	舒玛栎根系Q. shumardii root	靳微，2019
LS-TZS-1	Umbelopsis sp.	栎树根系Quercus sp. root	潘雪玉，2018

引物名称 Primer	序列（5’→3’） Sequences （5’→3’）	T_m值 T_m value/℃
PAL-F	GCACCCGACGCACTCAAAT	59.15
PAL-R	AAGGCCGTACCGTTCAAAATC	55.62
4CL-F	CCCTTCTGTGCTATTCTTCTGG	55.33
4CL-R	ATTGTTCTATCCTTGCCGTGTC	54.89
CHS-F	GATGTCCATTCGCCACGGTA	57.69
CHS-R	GAATGCCATAGCACCCTCAGA	57.18
bHLH-F	CAACCAGTGAAAGCGAACAAAG	54.75
bHLH-R	CAGAACCCGGAGCAAAATAG	53.53
α-tubulin-F	CCAGCAAGCGTTACCGATT	55.83
α-tubulin-R	TCCACGACGTCCATCGTTC	57.65

水平 Level	因素 Factors
水平 Level	A添加浓度 A concentration/（mg·mL^?1）	B添加时间 B add time/d	C静置时间 C stand time/d
?1	5	0	0
0	50	7	7
1	95	14	14

真菌诱导子类型 Types of fungal elicitors	A添加浓度 A concentration/(mg·mL^?1)	B添加时间 B adding time/d	C静置时间 C standing time/d	黄酮含量 Flavonoids content/(mg·g^?1)
NL-19M	7.89	0	9	3.45±0.16
NL-19ME	5	14	0	2.78±0.12
HSLFE	6.59	0	14	2.93±0.12

Fungal Elicitors Promote Flavonoid Biosynthesis in Sanghuangporus vaninii and the Underlying Mechanisms

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