利用HS-SPME-GC/MS法分析横坑切梢小蠹危害细叶云南松诱导抗性挥发物质

doi:10.11707/j.1001-7488.20210611

Abstract

Abstract:

Objective: This study aims to understand the harmfulness of bark beetle,Tomicus minor,and the stress response of Pinus yunnanensis var. tenuifolia,and explore the relationship between the response and metabolic volatiles related to resistance so as to put forward effective control measures. Method: The induced volatile substances of the pine were studied by headspace solid phase microextraction-gas chromatography/mass spectrometry with the shoots in different damage degrees in Yachang Forest Farm,Leye County,Guangxi. The optimum conditions of SPME were as follows: equilibrium for 15 min on solid phase extraction instrument at 75℃ and extraction for 15 min with 75 micron extraction head (DVB/CAR/PDMS). Result: The most volatile components were detected in severely damaged shoots,mainly terpenes and alcohols. The main metabolic volatile products of the pine were closely related to its damage degree. With the increase of the damage degree,the contents of α-pinene,3-pinene,β-caryophyllene and β-picengene increased,while the content of β-pinene decreased gradually,and only a small amount of longifolene was found in the control. In severely damaged shoots,β-pinene content was the lowest,only 47.85% of the control,while 3-pinene increased the most,higher than 62.83% of the control. The total amount of monoterpenoids and sesquiterpenoids oxygen-containing derivatives increased with the degree of damage,indicating that the insect resistance decreased with the increase of oxygen-containing compounds. Conclusion: The differences in the contents of 3-carene,β-pinene,β-elemene,β-caryophyllene and β-bichenylsolanee are the main reasons for inducing resistance of the pine to the bark beetle in transverse pits.Headspace solid phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS) is suitable for the rapid analysis of volatile components in the pine needles,and this study provides a scientific basis for the study of induced resistance substances of Tomicus minor.

Key words: Pinus yunnanensis, Tomicus minor, volatile chemicals, HS-SPME, GC/MS, discriminant analysis

CLC Number:

S763.30

Dongshan Wu,Jie Jia,Hu Chen,Peidong Yan,Rongxun Xu,Liuqin Yang,Zhangqi Yang. Analysis of Induced Resistant Volatile Compounds in Pinus yunnanensis var. tenuifolia Damaged by Tomicus minor (Coleoptera: Scolytidae)by HS-SPME-GC/MS Method[J]. Scientia Silvae Sinicae, 2021, 57(6): 103-110.

Figures/Tables 8

Fig.1

Fig.2

Fig.3

Fig.4

Fig.5

Table 1

GC-MS analysis of volatile components and relative contents in tender shoots of Pinus yunnanensis var. tenuifolia"

化合物Chemical compound	峰号 Peak No.	检出方式 Detection method	不同虫害梢挥发物 Volatile components from different damaged shoots(%)
化合物Chemical compound	峰号 Peak No.	检出方式 Detection method	A	B	C	D
顺-3-己烯-1-醇cis-3-hexen-1-ol	1	SR	0.021	0.017	0.024	0.015
三环烯Tricyclene	2	SR	0.201	0.224	0.196	0.255
α-蒎烯α-pinene	3	SR	20.894	21.668	22.582	20.361
莰烯Camphene	4	SR	0.802	0.779	0.896	0.778
桧烯Sabinene	5	SR	0.147	0.204	0.187	0.283
β-蒎烯β-pinene	6	SR	10.555	9.842	5.375	11.234
β-月桂烯β-myrcene	7	CS	0.608	0.611	5.821	0.588
α-水芹烯α-phellandrene	8	CS	0.094	0.115	0.075	0.072
顺-3-己烯醇乙酸酯cis-3-hexenyl acetate	9	SR	0	0.026	0.076	0
3-蒈烯3-carene	11	SR	3.883	4.026	5.849	3.592
α-松油烯α-terpinene	12	SR	0.087	0.114	0.098	0.125
对伞花烃p-cymene	13	SR	0.152	0	0.124	0.109
柠檬烯Limonene	15	CS	4.021	4.558	4.249	4.573
1, 8-桉叶素1, 8-cineole	16	CS	0.495	0.602	0.483	0.526
顺式-罗勒烯cis-ocimene	17	SR	0	0	0.015	0.051
反式-罗勒烯trans-ocimene	18	SR	0.004	0	0.009	0.027
γ-松油烯γ-terpinene	19	CS	0.198	0.185	0.117	0.202
异松油烯Terpinolene	22	SR	2.203	2.887	2.034	2.859
4-松油烯醇4-terpinenol	23	SR	0.462	0.447	0.489	0.511
α-松油醇α-terpineol	24	CS	0.688	0.732	0.709	0.825
马鞭草烯酮Verbenone	25	SR	0.061	0	0	0.033
乙酸芳樟酯Linalyl acetate	27	CS	0	0	0.025	0
乙酸龙脑酯Bornyl acetate	28	SR	0.895	0.744	0.784	0.721
α-毕澄茄烯α-cubebene	29	SR	0.209	0.187	0.203	0.219
α-乙酸松油酯α-terpinyl acetate	30	SR	0.011	0	0.019	0
可巴烯Copaene	31	SR	0.335	0.287	0.324	0.246
乙酸香叶酯Geranyl acetate	32	SR	0	0.029	0.047	0
β-榄香烯β-elemene	33	SR	4.955	5.238	4.019	5.488
长叶烯Longifolene	34	CS	0	0	0	0.123
β-石竹烯β-caryophyllene	35	SR	9.701	10.026	10.886	9.034
香树烯Aromadendrene	36	SR	0.258	0.276	0.184	0.298
α-石竹烯α-caryophyllene	38	CS	0.844	0.739	0.822	0.802
β-金合欢烯β-farnesene	41	SR	0.445	0.511	0.528	0.556
β-毕澄茄烯β-cubebene	42	SR	8.922	9.405	9.947	8.062
β-桉叶烯β-eudesmene	45	SR	0.666	0.602	0.689	0.722
γ-依兰油烯γ-muurolene	48	SR	0.049	0.067	0.083	0
甘香烯Elixene	49	SR	6.225	6.078	6.744	6.894
α-依兰油烯α-muurolene	55	SR	0.733	0.771	0.812	0.857
γ-杜松烯γ-cadinene	57	SR	5.011	4.883	4.252	5.013
1, 2, 4α, 5, 8, 8α-六氢化-4，7-二甲基-1-(1-甲基乙基)-萘1, 2, 4α, 5, 8, 8α-hexahydro-4, 7-dimethyl-1-(1-methylethyl)-naphthalene	58	SR	0.043	0.057	0	0.095
反式-澄花叔醇trans-nerolidol	59	SR	0	0	0.077	0.099
斯巴醇Spathulenol	61	SR	0.739	0.818	0.755	0.874
氧化石竹烯Caryophyllene	62	SR	0.438	0.367	0.512	0.430
δ-杜松醇δ-cadinol	64	SR	3.455	3.878	3.929	3.296
金合欢醇Farnesol	65	SR	0	0	0.067	0.089
乙酸金合欢酯Farnesyl acetate	66	SR	0.688	0.722	0.856	0.685
山达海松酸甲酯Methyl dehydaracopimarate	67	SR	0.022	0.031	0	0.095
脱氢枞酸甲酯Methyl dehydroabietate	68	SR	0	0.078	0.056	0

Table 1

Table 2

Table 3

References 0

	丁靖凯, 丁立生, 易元芬, 等. 滇产云南松、思茅松松针油的化学成分. 云南植物研究, 1987, 9 (4): 505- 508.
	Ding Q K , Ding L S , Yi Y F , et al. Chemical constituents of pine needle oil of Pinus yunnanensis and Pinus kesiya from Yunnan. Acta Botanica Yunnanica, 1987, 9 (4): 505- 508.
	戈峰, 李镇宇, 谢映平, 等. 我国主要松树诱导抗虫性的一些规律比较. 北京林业大学学报, 2002, 24 (3): 161- 165.
	Ge F , Li Z Y , Xie Y P , et al. Comparison of induced insect resistance of main pine trees in China. Journal of Beijing Forestry University, 2002, 24 (3): 161- 165.
	耿树香, 尹晓兵, 郑畹, 等. 云南3-蒈烯资源的调查研究. 林产化学与工业, 2006, 26 (4): 57- 60. doi: 10.3321/j.issn:0253-2417.2006.04.015
	Geng S X , Yin X B , Zheng W , et al. Investigation of 3-carene resources in Yunnan Province. Forest Products Chemistry and Industry, 2006, 26 (4): 57- 60. doi: 10.3321/j.issn:0253-2417.2006.04.015
	李聚英, 王军, 戴蕴青, 等. 香椿特征香气组成及其在贮藏中变化的研究. 北京林业大学学报, 2011, 6 (3): 127- 131.
	Li J Y , Wang J , Dai Y Q , et al. Study on characteristic aroma components of Toona sinensis and its changes during storage. Journal of Beijing Forestry University, 2011, 6 (3): 127- 131.
	李丽莎, 刘宏屏, 陈鹏, 等. 两种切梢小蠹生态学特性比较研究. 西部林业科学, 2006, 35 (1): 1- 5. doi: 10.3969/j.issn.1672-8246.2006.01.001
	Li L S , Liu H P , Chen P , et al. Comparative study on ecological characteristics of two species of tomicus. Western Forestry Science, 2006, 35 (1): 1- 5. doi: 10.3969/j.issn.1672-8246.2006.01.001
	孟昭军, 严善春, 徐伟. 长白落叶松8个家系挥发性化合物的比较分析. 林业科学, 2008, 44 (6): 91- 96. doi: 10.3321/j.issn:1001-7488.2008.06.016
	Meng Z J , Yan S C , Xu W , et al. Comparative analysis of volatile compounds in 8 families of Larix olgensis. Scientia Silvae Sinicae, 2008, 44 (6): 91- 96. doi: 10.3321/j.issn:1001-7488.2008.06.016
	宁眺, 樊建庭, 方宇凌, 等. 不同危害状态下寄主萜烯挥发物含量的变化及松墨天牛对其组分的触角电位反应. 昆虫学报, 2006, 49 (2): 179- 188. doi: 10.3321/j.issn:0454-6296.2006.02.003
	Ning T , Fan J T , Fang Y L , et al. Changes of terpene volatiles in different host plants and electroantennogram responses of Monochamus alternatus to its components. Acta Entomology, 2006, 49 (2): 179- 188. doi: 10.3321/j.issn:0454-6296.2006.02.003
	田玉红, 李梓, 梁才. 拉雅松和细叶云南松松针挥发油的化学成分. 中国实验方剂学杂志, 2012, 18 (1): 51- 55. doi: 10.3969/j.issn.1005-9903.2012.01.014
	Tian Y H , Li Z , Liang C . Chemical constituents of the essential oil from the needles of Pinus laja and Pinus yunnanensis. Chinese Journal of Experimental Prescriptions, 2012, 18 (1): 51- 55. doi: 10.3969/j.issn.1005-9903.2012.01.014
	吴东山, 唐鑫, 岑祖明, 等. 广西细叶云南松森林资源动态分析. 广西林业科学, 2016, 45 (3): 316- 321. doi: 10.3969/j.issn.1006-1126.2016.03.017
	Wu D S , Tang X , Cen Z M , et al. Dynamic analysis of forest resources of Pinus yunnanensis in Guangxi. Guangxi Forestry Science, 2016, 45 (3): 316- 321. doi: 10.3969/j.issn.1006-1126.2016.03.017
	徐长山, 张宏瑞, 张珍荫. 云南木蠹象的危险性分析. 中国森林病虫, 2004, 23 (4): 30- 32. doi: 10.3969/j.issn.1671-0886.2004.04.011
	Xu C S , Zhang H R , Zhang Z Y . Risk analysis of dendroctonus yunnanensis. Forest Diseases and Insect Pests in China, 2004, 23 (4): 30- 32. doi: 10.3969/j.issn.1671-0886.2004.04.011
	杨燕, 杨茂发, 杨再华, 等. 云南松松针的挥发性化学成分. 林业科学, 2009, 45 (5): 167- 173.
	Yang Y , Yang M F , Yang Z H , et al. Volatile constituents of Pinus yunnanensis needles. Scientia Silvae Sinicae, 2009, 45 (5): 167- 173.
	殷彩霞, 高竹林, 吕军, 等. 纵坑切梢小蠹对云南松梢提取趋性测试. 昆虫知识, 2002, 39 (5): 384- 386.
	Yin C X , Gao Z L , Lü J , et al. Test on the taxis of Tomicus piniperda to Pinus yunnanensis. Insect Knowledge, 2002, 39 (5): 384- 386.
	曾茜, 曹光群, 李明, 等. 顶空-固相微萃取/气相色谱-质谱联用法分析"无锡毫茶"中的香气成分. 分析测试学报, 2014, 33 (10): 1136- 1141. doi: 10.3969/j.issn.1004-4957.2014.10.007
	Zeng X , Cao G Q , Li M , et al. Analysis of aroma components in "Wuxi Haocha" by headspace solid phase microextraction/gas chromatography-mass spectrometry. Journal of Analytical Testing, 2004, 33 (10): 1136- 1141.
	郑宏. 松树营养物质及次生代谢物质含量与思茅松毛虫危害关系的判别分析. 生物灾害科学, 2013, 36 (1): 45- 52.
	Zheng H . Discriminant analysis for the relationship between the damaged degree of Dendrolimus kikuchii Matsumura and the contents of nutrition and secondary metabolites in pine needles. Biological Disaster Science, 2013, 36 (1): 45- 52.
	周楠, 毋亚梅, 张立新, 等. 松小蠹成虫对其聚集信息化合物的触角电位反应. 云南林业科技, 2000, 47 (3): 38- 42. doi: 10.3969/j.issn.1672-8246.2000.03.008
	Zhou N , Wu Y M , Zhang L X , et al. Electroantennogram responses of Dendroctonus punctatus adults to its aggregation information compounds. Forestry Science and Technology in Yunnan, 2000, 47 (3): 38- 42. doi: 10.3969/j.issn.1672-8246.2000.03.008
	Byers A , Lanne B S , Lfqvist J . Hosttree unsuitbility recognized by pine shoot beetles in flight. Experientia, 1987, 45, 489- 492.
	Carmona D , Lajeunesse M J , Johnson T J . Plant traits that predict resistance to herbivores. Functional Ecology, 2011, 25, 358- 367. doi: 10.1111/j.1365-2435.2010.01794.x
	Light D M , Knight A L . Specificity of codling moth (Lepidoptera: Tortricidae) for the host plant kairomone, ethyl (2E, 4Z)-2, 4-deca-dienoate: field bioassays with pome fruit volatiles, analogue and iso-meric compounds. Journal of Agriculture and Food Chemistry, 2005, 53 (10): 4046- 4053. doi: 10.1021/jf040431r
	Lou Y G , Cheng J A . Herbivore-induced plant volatiles: primary characteristics, ecological functions and its release mechanism. Acta Ecologica Sinica, 2000, 20 (6): 1097- 1106.
	Mu R M , Wang X R , Liu S X , et al. Rapid determination of volatile compounds in Toona sinensis (A. Juss.) Roem. by MAE-HS-SPME followed by GC-MS. Chromatographia, 2007, 65 (7): 463- 467.
	Paul W P , James H T . Plant volatiles as a defense against insect herbivores. Plant Physiology, 1999, 121 (6): 325- 331.
	Schroeder L M . Attraction of the bark beetle Tomicus piniperda to scots pine trees in relation to tree vigor and attack density. Entomologia Experimentalis et Applicata, 1987, 44 (12): 53- 58.
	Steffen A , Pawliszyn J . Analysis of flavor volatiles using head-space solid-phase microextraction. Journal of Agricultural and Food Chemistry, 1996, 44 (8): 2187- 2193. doi: 10.1021/jf950727k

危害程度 Degree of damage	α-蒎烯 α-pinene (X₁)	β-蒎烯 β-pinene (X₂)	3-蒈烯 3-carene (X₃)	柠檬烯 Limonene (X₄)	异松油烯 Terpinolene (X₅)	β-榄香烯 β-elemene (X₆)	β-石竹烯 β-caryophyllene (X₇)	β-毕澄茄烯 β-cubebene (X₈)	甘香烯 Elixene (X₉)	γ-杜松烯 γ-cadinene (X₁₀)	δ-杜松醇 δ-cadinol (X₁₁)
A	20.894±	10.555±	3.883±	4.021±	2.203±	4.955±	9.701±	8.922±	6.225±	5.011±	3.455±
A	0.525a	0.129ab	0.086a	0.039ab	0.071ab	0.095ab	0.045ab	0.033ab	0.051ab	0.027a	0.035a
B	21.668±	9.842±	4.026±	4.558±	2.887±	5.238±	10.026±	9.405±	6.078±	4.883±	3.878±
B	0.376ab	0.342b	0.055ab	0.086a	0.033a	0.055a	0.036bc	0.029bc	0.039ab	0.044ab	0.063ab
C	22.582±	5.375±	5.849±	4.249±	2.034±	4.019±	10.886±	9.947±	6.744±	4.252±	3.929±
C	0.093b	0.218c	0.048b	0.133ab	0.065ab	0.083c	0.018c	0.078c	0.022a	0.036ab	0.033ab
D	20.361±	11.234±	3.592±	4.573±	2.859±	5.488±	9.034±	8.062±	6.894±	5.013±	3.296±
D	0.275a	0.082a	0.107a	0.091a	0.173a	0.022a	0.061a	0.042a	0.014a	0.042a	0.057a

性状Character	Wilks’ Lambda	F	df₁	df₂	Sig.	Kruskal-Wallis Sig.
β-蒎烯β-pinene(X₂)	0.021	244.327	3	12	0	0.001 8
3-蒈烯3-carene(X₃)	0.035	217.055	3	12	0	0.002 5
β-榄香烯β-elemene(X₆)	0.022	224.871	3	12	0	0.003 7
β-石竹烯β-caryophyllene(X₇)	0.043	195.983	3	12	0	0.001 9
β-毕澄茄烯β-cubebene(X₈)	0.052	187.235	3	12	0	0.003 4

[1]	Qian Wang,Lili Shang,Tingting Yan,Yuan Chen,Yuejin Fu,Gaiyun Li. HPLC Fingerprint Characteristics of Agarwood from Different Origins [J]. Scientia Silvae Sinicae, 2021, 57(2): 150-159.
[2]	Jiayan Shen,Shuaifeng Li,Xiaobo Huang,Shaowu Wang,Jianrong Su. Ecological Resilience and Growth Degradation of Pinus yunnanensis at Different Altitudes in Jinsha River Basin [J]. Scientia Silvae Sinicae, 2020, 56(6): 1-11.
[3]	Li Yang, Xu Feiyun. Acoustic Emission Signal Characteristics of Pinus yunnanensis with Different Moisture Content [J]. Scientia Silvae Sinicae, 2019, 55(6): 96-102.
[4]	Fang Jiaxing, Wu Chengxu, Lu Wenjuan, Liu Fu, Zhang Sufang, Zhang Zhen, Kong Xiangbo. Correlation Analysis between the Shoot Damages and Trap Catches of Tomicus minor in the Pinus yunnanensis Forest [J]. Scientia Silvae Sinicae, 2019, 55(4): 129-135.
[5]	Chen Hongpeng, Peng Yan, Liu Guo, Li Hui, Gao Liqiong, Zhan Ni, Xie Yaojian. Dynamic Changes of Volatile Components from Developing Seeds of Plukenetia volubilis [J]. Scientia Silvae Sinicae, 2018, 54(9): 157-168.
[6]	Xu Meng, Zhang Jingwei, Wu Lingshang, Liu Jingjing, Si Jinping, Zhang Xinfeng. Determination of Volatile Components from Chimonanthus Flowers by HS-SPME-GC-MS [J]. Scientia Silvae Sinicae, 2016, 52(12): 58-65.
[7]	Li Chaochan, Yi Yin, Quan Wenxuan, Tian Honghong. The Natural Volatile Components of Allelochemicals in the Wild Alpine Rhododendron Community [J]. Scientia Silvae Sinicae, 2015, 51(12): 35-44.
[8]	Lin Yinghua, Jia Xudong, Xu Yanpeng, Li Huiren, Liu Xueshuang, Xu Yongbo, Wei Changlei, Liu Sanzhang, Wang Lizhong. Ground-Dwelling Soil Animal Community and Niche Analysis of A Typical Forest Swamp in Daxing'anling Mountains [J]. Scientia Silvae Sinicae, 2015, 51(12): 53-62.
[9]	Liang Wei;Cheng Jun;Bu Chunya;Jin Yongsheng;Shi Guanlu;Wang Younian;. Componential Analysis and Acaricidal Activities of Stellera chamaejasme Extracts by Supercritical Fluid Extraction [J]. Scientia Silvae Sinicae, 2012, 48(1): 181-185.
[10]	Yin Yanbao;Tang Shouzheng;Lang Pumei;Gao Ruixin. Determination of DEM Based on Multiple-Echo Data of Airborne LiDAR in Mountainous Wooded Area Using Discriminant Analysis [J]. Scientia Silvae Sinicae, 2011, 47(12): 106-113.
[11]	Li Hongqun;Lian Zhenmin;Chen Cungen. Selection of Winter Dust-Bathing Sites by Brown-Eared Pheasant in Huanglong Mountains Nature Reserve, China [J]. Scientia Silvae Sinicae, 2011, 47(11): 93-98.
[12]	Du Xiujuan;Song Liwen;Gao Changqi;Zhou Chunyan;Li Xingpeng;Mao Baoju;Gao Qunzong;Pan Liming. Volatiles from Pinus koraiensis at Different Stages after Infested by Dioryctria pryeri and the Relationship with Host Selection [J]. Scientia Silvae Sinicae, 2010, 46(8): 106-113.
[13]	Yin Yanlei;Yuan Zhaohe;Feng Lijuan;Zhao Xueqing;Wang Jinzheng;Wang Chao. GC/MS Analysis of Aromatic Components in Katy Apricot Fruit in Various Developmental Periods under Different Cultivation Condition [J]. Scientia Silvae Sinicae, 2010, 46(7): 92-98.
[14]	Jiang Maosheng;Xu Wenyao;Lin Wei;Zheng Xinyu;Huang Biao;Chen Lihui. Inhibition Activities of Bamboo-Based Tar against the Plant Pathogen Fungi [J]. Scientia Silvae Sinicae, 2010, 46(4): 93-97.
[15]	Zhang GuogangZhang ZhengwangYang FengyingLi Shiguang. Habitat Selection of Brown-Eared Pheasant at the Wulushan National Nature Reserve of Shanxi, China [J]. Scientia Silvae Sinicae, 2010, 46(11): 100-103.

Analysis of Induced Resistant Volatile Compounds in Pinus yunnanensis var. tenuifolia Damaged by Tomicus minor (Coleoptera: Scolytidae)by HS-SPME-GC/MS Method

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 8

References 0

Related Articles 15

Recommended Articles

Metrics

Comments