Abstract:

Composition and relative contents of volatiles from different tissues of Pinus koraiensis at different stages after infested by Dioryctria pryeri by using TCT-GC/MS technique and olfactory response of larvae to the volatiles were further analyzed. The result showed that there were differences in volatile composition and contents from the pine cones infested by D. pryeri and the other different tissues infested by the pest larvae in overwinter at different infesting stages. Olfactory response of overwinter larvae indicated that the simulated overwinter larvae obviously prefered alcohol solution with standard sample of main volatile components from healthy cones, healthy branches and needles to alcohol solvent; and the larvae had an obvious orientation to healthy cones compared with the branches and needles. In addition, they had an orientation to alcohol solution with α-pinene, limonene, α-pinene+β-pinene compared with standard sample of main volatile components from healthy cones of Pinus koraiensis, among which α-pinene had the strongest attract ability.

Key words: Dioryctria pryeri, Pinus koraiensis, cones, volatiles, TCT-GC/MS, olfactory response