麻竹同源异型盒基因DlKNOX1的克隆及功能初步分析

Abstract

Abstract:

Homeobox genes play important roles in determining plant morphogenesis. A homeobox gene was isolated from Dendrocalamus latiflorus with RT-PCR and RACE methods. The full length of the cDNA was 1 452 bp which would encode a 330-aa protein containing one KNOX1 domain, one KNOX2 domain, one ELK domain and one Homeobox domain, indicating that it belongs to KNOX protein, and the gene was designed as DlKNOX1 . Tissue specific expression showed that DlKNOX1 expressed in knob with the highest level, followed by in stem and sheath, and the lowest in leaf. DlKNOX1 gene was constructed into the multiple cloning sites of pPZP vector driven by a 35S promoter, and transferred into Arabidopsis thaliana. RT-PCR analysis showed that DlKNOX1 was expressed in the transgenic plants. The phenotype of transgenics showed that flowering time was later than that of wild type, and the pods position changed obviously, which suggested that DlKNOX1 might participate in the regulation of D. latiflorus morphogenesis.

Key words: Dendrocalamus latiflorus, homeobox gene, tissue specific expression, ectopic expression, morphogenesis

CLC Number:

S718.46

Liu Qing, Wang Yufeng, Zhao Hansheng, Chen Ying, Gao Zhimin. Molecular Characteristics and Primary Functional Analysis of DlKNOX1 Gene from Dendrocalamus latiflorus[J]. Scientia Silvae Sinicae, 2014, 50(2): 56-62.

References

李春苑, 阮美煜,贾海燕,等. 2009. 同源异型盒基因I类KNOX的表达调控及在植物形态建成中的作用. 细胞生物学杂志,31(5): 635-640.
(Li C Y, Ruan M Y, Jia H Y, ,et al. 2009. The expression and regulation of class I KNOX of homeobox genes and their functions in plant morphogenesis. Chinese Journal of Cell Biology, 31(5): 635-640. [in Chinese] )
熊文愈,丁祖福,李又芬. 1980. 竹类植物的居间分生组织与节间生长Ⅰ: 秆茎的居间分生组织与节间生长. 林业科学,16(2): 81-89.
(Xiong W Y, Ding Z F, Li Y F. 1990. Intercalary meristem and internodal elongation of bamboo plants. Scientia Silvae Sinicae, 16(2): 81-89. [in Chinese] )
徐芳,熊爱生,彭日荷,等. 2005. 植物遗传转化的新方法: Floral Dip. 中国蔬菜,(3): 29-31.
Castellano M M, Sablowski R. 2005. Intercellular signalling in the transition from stem cells to organogenesis in meristems. Curr Opin Plant Biol, 8(1): 26-31.
Finn R D, Mistry J, Tate J, et al. 2010. The Pfam protein families database. Nucleic Acids Res, 38(Database issue): D211-D222.
Frugis G, Giannino D, Mele G, et al. 2001. Overexpression of KNAT 1 in lettuce shifts leaf determinate growth to a shoot-like indeterminate growth associated with an accumulation of isopentenyl-type cytokinins. Plant Physiol, 126(4): 1370-1380.
Gao Zhimin, Li Xueping, Li Lubin, et al. 2006. An effective method for total RNA isolation from bamboo. Chinese Forest Sci Tech, 5(3): 52-54.
Gehring W J, Affolter M, Bürglin T. 1994. Homeodomain proteins. Annu Rev Biochem, 63: 487-526.
Hareven D, Gutfinger T, Parnis A, et al. 1996. The making of a compound leaf: genetic manipulation of leaf architecture in tomato. Cell, 84(5): 735-744.
Hay A, Barkoulas M, Tsiantis M. 2006. ASYMMETRIC LEAVES 1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis. Development, 133(20): 3955-3961.
Hay A, Tsiantis M. 2010. KNOX genes: versatile regulators of plant development and diversity. Development, 137(19): 3153-3165.
Hewelt A, Prinsen E, Thomas M, et al. 2000. Ectopic expression of maize KNOTTED 1 results in the cytokinin-antotrophic growth of cultured tobacco tissues. Planta, 210(6): 884-889.
Hu X, Wu Q F, Xie Y H, et al. 2005. Ectopic expression of the PttKN 1 gene induces alterations in the morphology of the leaves and flowers in Petunia hybrida Vilm. J Integr Plant Biol, 47(10): 1153-1158.
Janssen B J, Lund L, Sinha N. 1998. Overexpression of a homeobox gene, LeT 6 , reveals indeterminate features in the tomato compound leaf. Plant Physiol, 117(3): 771-778.
Kerstetter R, Vollbrecht E, Lowe B, et al. 1994. Sequence analysis and expression patterns divide the maize KNOTTED 1 -like homeobox genes into two classes. Plant Cell, 6(12): 1877-1887.
Lincoln C, Long J, Yamaguchi J, et al. 1994. A KNOTTED 1 -like homeobox gene in Arabidopsis is expressed in the vegetative meristem and dramatically alters leaf morphology when overexpressed in transgenic plants. Plant Cell, 6(12): 1859-1876.
Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2^-ΔΔCt method. Methods, 25(4): 402-408.
Ma H, McMullen M D, Finer J J. 1994. Identification of a homeobox containing gene with enhanced expression during soybean (Glycine max L.) somatic embryo development. Plant Mol Biol, 24(3): 465-473.
Mallo M, Wellik D M, Deschamps J. 2010. Hox genes and regional patterning of the vertebrate body plan. Dev Biol, 344(1): 7-15.
Matsuoka M, Ichikawa H, Saito A, et al. 1993. Expression of a rice homeobox gene causes altered morphology of transgenic plants. Plant Cell, 5(9): 1039-1048.
Meisel L, Lam E. 1996. The conserved ELK-homeodomain of KNOTTED-1 contains two regions that signal nuclear localization. Plant Mol Biol, 30(1): 1-14.
Mukherjee K, Brocchieri L, Bürglin T R. 2009. A comprehensive classification and evolutionary analysis of plant homeobox genes. Mol Biol Evol, 26(12): 2775-2794.
Müller K, Romano N, Gerstner O, et al. 1995. The barley Hooded mutation caused by a duplication in a homeobox gene intron. Nature, 374(72): 7-30.
Nagasaki H, Sakamoto T, Sato Y, et al. 2001. Functional analysis of the conserved domains of a rice KNOX homeodomain protein, OSH15. Plant Cell, 13(9): 2085-2098.
Ori N, Juarez M T, Jackson D, et al. 1999. Leaf senescence is delayed in tobacco plants expressing the maize homoobox gene KNOTTED 1 under the control of a senescence-activated promoter. Plant Cell, 11(6): 1073-1080.
Scofield S, Murray J A. 2006. KNOX gene function in plant stem cell niches. Plant Mol Biol, 60(6): 929-946.
Sentoku N, Sato Y, Kurata N, et al. 1999. Regional expression of the rice KN 1 -type homeobox gene family during embryo, shoot and flower development. Plant Cell, 11(9): 1651-1664.
Sinha N R,Williams R E, Hake S. 1993. Overexpression of the maize homeobox gene, KNOTFED- 1 , causes switch from determinate to indeterminate cell fates. Genes Dev, 7(5): 787-795.
Tamaoki M, Kusaba S, Kano-Murakami Y, et al. 1997. Ectopic expression of a tobacco homeobox gene, NTH 15 , dramatically alters leaf morphology and hormone levels in transgenic tobacco. Plant Cell Physiol, 38(8): 917-927.
Vollbrecht E, Veit B, Sinha N, et al. 1991. The developmental gene Knotted- 1 is a member of a maize homeobox gene family. Nature, 350(6315): 241-243.
Williams R W. 1998. Plant homeobox genes: many functions stem from a common motif. Bioessays, 20(4): 280-282.

[1]	Xu Xiangting, Wang Lin, Huang Ping, Wan Yuxuan, Li Jianzhong, Wan Xianchong. Effects of Wind and Irradiation on Growth and Morphogenesis of Platycladus orientalis and Quercus variabilis [J]. Scientia Silvae Sinicae, 2014, 50(7): 164-168.
[2]	Yang Li, Chen Dongliang, Peng Zhenhua, Zhao Hansheng, Gao Zhimin. Cloning of Transcription Factor DlSCL6 from Dendrocalamus latiflorus and Its Ectopic Expression in Arabidopsis thaliana [J]. Scientia Silvae Sinicae, 2014, 50(11): 52-57.
[3]	Jiang Yao;Qi Xiaoli;Tang Fang;Zhao Shutang;Chen Jun;Lu Mengzhu. Application of Genechip in Screening the Stem Differentiation-Related Genes [J]. Scientia Silvae Sinicae, 2012, 48(11): 42-48.
[4]	Gao Zhimin;Liu Qing;Mu Shaohua;Li Xueping;Hu Tao. Cloning and Primary Functional Analysis of a Zinc Finger Protein Gene of BoBZF from Bambusa oldhamii [J]. Scientia Silvae Sinicae, 2012, 48(10): 49-54.
[5]	Peng Jiang;Sun Yiming;Song Feng;Li Peng;Rao Can;Sun Min. Molecular Cloning and Prokaryotic Expression of the Gene Encoding HMG-CoA Reductase from Eucalyptus urophylla×E.grandis [J]. Scientia Silvae Sinicae, 2011, 47(2): 164-168.
[6]	Gao Zhimin;Yang Xuewen;Peng Zhenhua;Li Xueping;Mu Shaohua;Ma Yanjun. Molecular Characterization and Subcellular Localization of BoSUT2 from Bambusa oldhamii [J]. Scientia Silvae Sinicae, 2010, 46(2): 45-50.
[7]	Gao Zhimin;Zheng Bo;Peng Zhenhua;. Isolation of PeMADS1 Gene from Phyllostachys edulis and Its Transformation in Arabidopsis thaliana [J]. Scientia Silvae Sinicae, 2010, 46(10): 37-41.
[8]	Gao Zhimin;Liu Cheng;Liu Yingli;Peng Zhenhua. Cloning and High Prokaryotic Expression of the Light Harvesting Chlorophyll a／bBinding Protein Gene from Bamboo(Phyllostachys edulis) [J]. Scientia Silvae Sinicae, 2009, 12(3): 145-149.
[9]	Li Shimei;Xing Shiyan;Li Baojin;Wang Li. Review on Ontogeny and Phylogeny of Ginkgo biloba var.epiphylla and Its Genesis Mechanism [J]. Scientia Silvae Sinicae, 2007, 43(05): 90-98.
[10]	Zhan Yaguang;Yang Chuanping;Wang Yucheng. ANATOMICAL STUDY ON MORPHOGENESIS IN VITRO CULTURE FROM BETULA PLATYPHYLLA [J]. Scientia Silvae Sinicae, 2002, 38(6): 142-145.

Molecular Characteristics and Primary Functional Analysis of DlKNOX1 Gene from Dendrocalamus latiflorus

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 10

Recommended Articles

Metrics

Comments