Welcome to visit Scientia Silvae Sinicae，Today is

Scientia Silvae Sinicae ›› 2009, Vol. 12 ›› Issue (3): 145-149.doi: 10.11707/j.1001-7488.20090325

Previous Articles     Next Articles

Cloning and High Prokaryotic Expression of the Light Harvesting Chlorophyll a／bBinding Protein Gene from Bamboo(Phyllostachys edulis)

Gao Zhimin1,Liu Cheng2,Liu Yingli1,Peng Zhenhua3   

  1. (1. Key Laboratory of Bamboo and Rattan Science and Technology of State Forestry Administration International Center for Bamboo and Rattan Beijing 100102; 2.Institute of Botany, the Chinese Academy of Sciences Beijing 100093; 3. Research Institute of Forestry, Chinese Academy of Forestry Beijing 100091)
  • Received:2008-03-13 Revised:1900-01-01 Online:2009-03-25 Published:2009-03-25

PDF (PC)

707

Abstract:

Abstract： The light harvesting chlorophyll a／bbinding protein is one of key proteins in the transformation from light energy to chemical energy. An open reading frame coding precursor protein of cab gene was cloned from the first strand of bamboo cDNA through RTPCR methods, and named as cabPhE1 (cab gene 1 from Phyllostachys edulis EF207229). The sequence analysis showed that the deduced polypeptide was highly homologous to some other CAB proteins from monocotyledon, and the gene belonged to lhcb2 family. Tissue specific expression showed that cabPhE1expressed higher in leaf than sheath and stem. The prokaryotic expression vector of cabPhE1gene encoding the mature protein was constructed by subcloning the fragment into pET23 a and was expressed in Escherichia coli induced by IPTG. The molecular weight of the induced protein was about 28 ku，approximate to that of the mature protein. This work is a key to the further research on in vitro reconstitution of lightharvesting Chl a/b complexes.

Key words: Key words： bamboo(Phyllostachys edulis), light harvesting chlorophyll a／bbinding protein gene(cab), tissue specific expression, prokaryotic expression