微量元素对雷公藤不定根生长和次生代谢产物含量的影响

Abstract

Abstract:

In this study, the effects of different concentration trace elements in NT medium on adventitious root growth, the accumulation of total alkaloid and triptolide of Tripterygium wilfordii were investigated. The results showed that the trace elements of Mn,Zn,Cu,Co,B,I and Mo influenced the adventitious root growth and the accumulation of total alkaloid and triptolide. The optimal NT medium trace elements for the growth of adventitious root were 2 times the concentration of MnSO₄,with concentrations of ZnSO₄,CuSO₄,CoCl₂,H₃BO₃ and KI maintained unchanged and 2 times concentrations of Na₂MoO₄.As for the accumulation of total alkaloids, the concentration of KI had no impact on the accumulation of total alkaloids, 2 times the concentration of CoCI₂ and CuSO₄, removal of H₃BO₃ and Na₂MoO₄, and 4 times concentrations of MnSO₄ and ZnSO₄ were suitable. In NT medium, 2 times the concentration of ZnSO₄ and CuSO₄, removal of H₃BO₃, Na₂MoO₄ and CoCl₂, and 4 times concentrations of MnSO₄ and KI were optimal for the accumulation of triptolide.

Key words: Tripterygium wilfordii, adventitious root, tissue culture, trace elements, secondary metabolites

CLC Number:

S817.73

Li Yan, Wen Pengfei, Cui Lei, Lei Jiamin, Zhang Xing. Effects of Trace Elements on Adventitious Root Growth and Secondary Metabolites Content of Tripterygium wilfordii[J]. Scientia Silvae Sinicae, 2014, 50(2): 134-138.

References

高柳青,田长彦,胡明芳. 1999.土壤锌、锰胁迫对棉花氮、磷养分吸收的影响.资源科学,21(3): 72-76.
(Gao L Q, Tian C Y, Hu M F. 1999. The impact of soil zinc and manganese stress on cotton absorption of nutrient nitrogen and phosphorus. Resources Science, 21(3): 72-76. [in Chinese] )
贺学礼.2009.植物生物学.北京: 科学出版社,161-166.
李春雷,崔国新,许志茹,等.2009.植物二氢黄酮醇4-还原酶基因的研究进展.生物技术通讯, 20(3): 442 -445.
(Li C L, Cui G X, Xu Z R, et al. 2009. Research advances on dihydroflavonol 4-reductase gene. Letters in Biotechnology, 20(3): 442 -445. [in Chinese] )
李琰,陈培,杨钰琪,等.2012.雷公藤组培产物的杀虫杀菌活性研究.西北植物学报,32(10): 2116-2121.
(Li Y, Chen P, Yang Y Q, et al. 2012. Insecticidal and antifungal activity of Tripterygium wilfordii cultures. Acta Botanica Boreali-Occidentalia Sinica, 32(10): 2116-2121. [in Chinese] )
李琰,冯俊涛,史晓燕,等.2009.雷公藤组培产物中雷公藤甲素和总生物碱含量的测定.农药学学报,11(3): 367-372.
(Li Y, Feng J T, Shi X Y, et al. 2009. Study on the determination of triptolide and total alkaloids in Tripterygium wilfordii hook. f. cultures. Chinese Journal of Pesticide Science, 11(3): 367-372. [in Chinese] )
李琰,冯俊涛,王永宏,等.2010. 培养基及培养条件对雷公藤愈伤组织生长和次生代谢产物含量的影响.林业科学,46(5): 64-69.
(Li Y, Feng J T, Wang Y H, et al. 2010. Effects of basic media and culture conditions on callus growth and secondary metabolite content of Tripterygium wilfordii. Scientia Silvae Sinicae, 46(5): 64-69. [in Chinese] )
李琰,杨钰琪,陈培,等.2013.培养基成分对雷公藤不定根生长和次生代谢产物含量的影响.林业科学,49(5): 71-76.
(Li Y, Yang Y Q, Chen P, et al. 2013. Effects of medium components on the growth and secondary metabolites contents in adventitious roots of Tripterygium wilfordii. Scientia Silvae Sinicae, 49(5): 71-76. [in Chinese] )
李雪飞,胡静静,王庆菊,等.2010.微量元素喷施对紫叶桃叶片花色苷含量及其合成酶活性的影响.林业科学,46(12): 75-79.
(Li X F, Hu J J, Wang Q J, et al. 2010. Effects of spraying microelement on anthocyanin and the relevant biosynthesis enzymes in Prunus persica f. atropurpurea leaves. Scientia Silvae Sinicae, 46(12): 75-79. [in Chinese] )
刘铮. 1994.植物微量元素化学.北京: 农业出版社,171-192.
罗都强,秦建春,张兴.2001.雷公藤甲素对粘虫中肠消化酶及其组织结构的影响.西北农林科技大学学报: 自然科学版,29(6): 57-60.
(Luo D Q, Qin J C, Zhang X. 2001. Effects of triptolide on the midgut tissue and the digestive enzyme activities of Mythimna separata (Walker) larvae. Journal of Northwest Sci-Tech University of Agriculture and Forestry, 29(6): 57-60. [in Chinese] )
尚富德,马云峰.2003.不同浓度的La³⁺和Cu²⁺对银杏固体培养和液体培养组织中黄酮产量的影响.西北植物学报,23(9): 1577-1580.
(Shang F D, Ma Y F. 2003. The effect of different concentrations of La³⁺ and Cu²⁺ on flavonoid production in solid and liquid culture of Ginkgo biloba L. Acta Botanica Boreali-occidentalia Sinica, 23(9): 1577-1580. [in Chinese] )
施益华,刘鹏. 2003.锰在植物体内生理功能研究进展.江西林业科技,(2): 26-28.
(Shi Y H, Liu P. 2003. Review of advance in physiological function of manganese in plants. Jiangxi Forestry Science and Technology, (2): 26-28. [in Chinese] )
张兴,李琰,冯俊涛,等.2008.一种由雷公藤悬浮细胞诱导不定根的方法.中国专刊,ZL200810017697.8.
仲剑,孙志华,吴强,等.2009.雷公藤甲素对人子宫内膜癌抑制作用的裸鼠体内研究.南京医科大学学报: 自然科学版,29(6): 775-778.
周琳,马志卿,冯俊涛,等.2006.雷公藤生物碱制品对小菜蛾和菜青虫的控制效果.西北农林科技大学学报: 自然科学版,34(12): 169-173.
(Zhou L, Ma Z Q, Feng J T, et al. 2006. Effects of triptolide on the midgut tissue and the digestive enzyme activities of Mythimna separata (Walker) larvae. Journal of Northwest Sci-Tech University of Agriculture and Forestry, 34(12): 169-173. [in Chinese] )
Brinker A M, Raskin I. 2005. Determination of triptolide in root extracts of Tripterygium wilfordii by solid-phase extraction and reverse-phase high-performance liquid chromatography. Journal of Chromatography A, 1070(1/2):65-70.
Giri A, Dhingra V, Giri C C, et al. 2001. Biotransformations using plant cells, organ cultures and enzyme systems: current trends and future prospects. Biotechnol Advances, 19(3):175-199.
Gonzalez A G,Jimenez I A,Ravelo A G,et al. 1997. Antifeedant activity of sesquiterpenes from Celastraceae.Biochemical Systematics and Ecology, 25(6): 513-519.
Huang W, He T, Chai C, et al. 2012. Triptolide inhibits the proliferation of prostate cancer cells and down-regulates SUMO-specific protease 1 expression. PLoS ONE, 7(5): e37693.
Johnson S M, Wang X, Evers B M. 2011. Triptolide inhibits proliferation and migration of colon cancer cells by inhibition of cell cycle regulators and cytokine receptors. Journal of Surgical Research, 168(2): 197-205.
Kutney J P, Choi L S, Duffin R, et al. 1983. Cultivation of Tripterygium wilfordii tissue cultures for the production of the cytotoxic diterpene tripdiolide. Planta Medica, 48(7):158-163.
Liu Q. 2011. Triptolide and its expanding multiple pharmacological functions. International Immunopharmacology, 11(3): 377-383.
Nakano K, Yoshida C, Furukawa W, et al. 1998. Terpenoids in transformed root culture of Tripterygium wilfordii.Phytochemistry,49(6): 1821-1824.
Perotti J C, Silva Rodrigues I C, Kleinowski A M, et al. 2010. Produçáo de betacianina em erva-de-jacaré cultivada in vitro com diferentes concentrações de sulfato de cobre. Ciência Rural, Santa Maria, 40(9): 1874-1880.

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Effects of Trace Elements on Adventitious Root Growth and Secondary Metabolites Content of Tripterygium wilfordii

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