耐盐基因Bet-A转化小黑杨的研究

doi:10.11707/j.1001-7488.20010607

Scientia Silvae Sinicae ›› 2001, Vol. 37 ›› Issue (6): 34-38.doi: 10.11707/j.1001-7488.20010607

Previous Articles Next Articles

STUDY ON THE TRANSFORMATION OF POPULUS SIMONII×P.NIGRA WITH SALT RESISTANCE GENE BET A

Yang Chuanping,Liu Guifeng,Liang Hongwei,Zhang Hui

Northeast Forestry University Harbin150040;Shandong Teachers University Jinan250014

Received:2001-06-25 Revised:1900-01-01 Online:2001-11-25 Published:2001-11-25

Abstract

Abstract:

In this study,exogenous gene Bet-A (encoding choline dehydrogenase,synthesizing glycine betaine) was introduced into Populus simonii×P.nigra by Agro-bacterium tumefacien,so as to enhance salt resistance in transformed plant.Firstly,the optimal media of P.simonii×P.nigra for bud differentiation and rooting were established.The sensitivity of the explants to Kanamycin was tested in order to decide a reasonable selective pressure for transformation.Secondly,Bet-A gene was introduced into leaves of asepsis plants of poplar by agrobacterium,and then transformed poplar plants were obtained.PCR and southern blotting analyses showed that Bet-A gene has been integrated into the genome of P.simonii×P.nigra.

Key words: Glycine betaine, Bet-A gene, Genetic transformation, Populus simonii×, P.nigra

Yang Chuanping;Liu Guifeng;Liang Hongwei;Zhang Hui. STUDY ON THE TRANSFORMATION OF POPULUS SIMONII×P.NIGRA WITH SALT RESISTANCE GENE BET A[J]. Scientia Silvae Sinicae, 2001, 37(6): 34-38.

[1]	Yingnan Chen,Chuanjing Hu,Qiang Zhuge,Jianjun Hu,Tongming Yin. Thirty Years of Agrobacterium-Mediated Genetic Transformation of Populus [J]. Scientia Silvae Sinicae, 2022, 58(12): 114-129.
[2]	Yunxin Cen,Jia Liu,Faju Chen,Jingyuan Yang,Qiang Liu,Tao Wang,Hongwei Liang. Agrobacterium-Mediated Genetic Transformation System of Catalpa bungei [J]. Scientia Silvae Sinicae, 2021, 57(8): 195-204.
[3]	Xiaohong Yang,Xiaoyang Chen. Transformation of Lespedeza bicolor with Bivalent Gene xylA & BADH under Xylose Selection System [J]. Scientia Silvae Sinicae, 2021, 57(6): 74-84.
[4]	Bi Liang,Jiaqi Zhang,Fei Ren,Hengkang Hu,Chuanmei Xu,Yuanyuan Hu,Youjun Huang,Heqiang Lou,Qixiang Zhang. Cloning and Expression Analysis of Ent-Kaurene Oxidase Gene CcKo in Carya cathayensis [J]. Scientia Silvae Sinicae, 2020, 56(10): 70-82.
[5]	Gu Zhanying, Yang Ruonan, Chen Hao. The Establishment of Isolation and Transient Transformation Methods of Protoplasts of Vernicia fordii Mesophyll Cells [J]. Scientia Silvae Sinicae, 2018, 54(1): 46-53.
[6]	Cheng Zhenxia, Hu Haitao, Yang Li, Wang Changchun, Guo Weidong, Yang Ling. Overexpression of EutPDS Gene from Elaeagnus umbellata Increases Lycopene Content in Tomato Fruit [J]. Scientia Silvae Sinicae, 2017, 53(1): 62-69.
[7]	Luo Zaiqi, Guo Huili, Yang Yadong, Yang Mingfeng, Ma Lanqin, Wang Younian. Agrobacterium-Mediated Transformation of Resveratrol Synthase Gene (PcPKS5) into Huping Jujube (Zizyphus jujuba) [J]. Scientia Silvae Sinicae, 2015, 51(10): 101-109.
[8]	Zhang Weixi, Liu Boyang, Ding Changjun, Zhang Bingyu, Huang Qinjun, Chu Yanguang, Su Xiaohua. Transformation of Zinc Finger Protein Transcription Factor Gene (ZxZF) and Preliminary Evaluation of Drought Tolerance in Populus × euramericana [J]. Scientia Silvae Sinicae, 2014, 50(3): 31-37.
[9]	Li Simeng;Wang Yonglin;Huang Donghui;Tian Chengming. Establishment of a PEG-Mediated Genetic Transformation System and Expression of Green Fluorescence Protein in Colletotrichum gloeosporioides [J]. , 2013, 49(5): 121-127.
[10]	Ouyang Lejun;Liu Yuan;Huang Zhenchi;Zeng Fuhua. Transformation of TSRF 1 into Eucalyptus urophylla and the Broad-Spectrum Disease Resistance of the Transgenic Plant to Diseases [J]. Scientia Silvae Sinicae, 2013, 49(4): 46-53.
[11]	Li Xiaorui;Hu Shanglian;Cao Ying;Lu Xueqin;Ren Peng;Wu Xiaoyu;Zhou Meijuan. Agrobacterium-Mediated Transformation of 4CL Gene from Neosinocalamus affinis into Dendrocalamus farinosus [J]. Scientia Silvae Sinicae, 2012, 48(3): 38-44.
[12]	Jia Xiaoming;Zhang Huanling;Fan Junfeng. System Optimization of Precociously Flowering of Poplar Induced by FT Gene Controlled by a Heat Shock Promoter [J]. Scientia Silvae Sinicae, 2011, 47(11): 37-43.
[13]	Zhang Xiaoying;Gan Jing;Yin Weilun;Zhu Zhen;Wang Huafang. Transformation of the Insecticidal Agglutinin Gene(gna) from Snowdrop (Galanthus nivalis) into Sophora japonica and Resistance of the Transgenic Plants to Aphids [J]. Scientia Silvae Sinicae, 2010, 46(2): 51-55.
[14]	Song Enhui;Cai Cheng;Wei Guo;Gao Hui;Xiang Yan. Cultivation of Low Lignin Poplar by RNA Interference [J]. Scientia Silvae Sinicae, 2010, 46(2): 39-44.
[15]	Zhang Xiaoying;Gan Jing;Yin Weilun;Zhu Zhen;Wang Huafang. An effective Transformation System of Sophora japonica [J]. Scientia Silvae Sinicae, 2009, 12(5): 20-26.

STUDY ON THE TRANSFORMATION OF POPULUS SIMONII×P.NIGRA WITH SALT RESISTANCE GENE BET A

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments