THE SEQUENCE VARIATION OF MITOCHONDRIAL GENES IN PINUS
Mengzhu Lu1,E.Szmidt Alfred2
1. Research Institute of Forestry, Chinese Academy of Forestry Beijing 100091 2. Department of Forest Genetics and Plant Physiology, SLU, 90183, Ume?, Sweden
The partial sequencing pattern of three genes The arrows indicate the position of the nucleotide substitutions; PCO : P. contorta; PKW: P. kwangtungensis; PME: P. merkusii; PPA: P. parviflora. "
Fig.1
Fig.2
Sequence alignment of the partial atp6 fragments from 12 Pinus species "*" indicates the same nucleotide as the given sequence shown; PNI: Pinus nigra; PCO : P. contorta; PDE: P. densata; PTA : P. tabulaeform is; PYU: P. yunnanensis; PME: P. merkusii; PSY : P. sylvestris; PMA : P. massoniana; PKW: P. kwangtungensis; PPE: P. peuce; PGR: P. griffithii; PPA: P. parviflora. "
Fig.2
Fig.3
The neighbor-joining tree for 12 Pinus species based on their atp6 sequences"
Fig.3
Fig.4
Sequence alignment of the partial orf25 fragments from 12 Pinus species"
Fig.4
Fig.5
The neighbor-joining tree for 12 Pinus species based on their orf25 sequences"
Fig.5
Fig.6
The neighbor-Joining tree for 12 Pinus species based on the joined atp6 and orf25 sequences"
Doyle J J , Doyle J L . A rapid DNA isolation procedure for small quantities of fresh tissue. Phytochem. Bull., 1987. 19, 11- 15.
Glaubitz J C , Carlson J E . RNA editing in the mitochondria of a conifer. Curr. Genet., 1992. 22, 163- 165.
Karpinska B , Karpinski S , Hällgren J E . The genes encoding subunit 3 of NADH dehydrogenase and ribosomal protein S 12 are co-transcribed and edited in Pinus sylvestris (L.) mitochondria. Current Genetics., 1995. 28, 423- 428.
Lu M-Z , Wang X-R , Szmidt A E . RNA editing and its effects on the evolution of coxI gene in gymnosperms. Plant Molecular Biology., 1998. 37, 225- 234.
Mirov N T . The genus Pinus. New York: The Ronald Press Company. 1967.
Palmer J D. Mitochondrial DNA in plant systematics: applications and limitations. In Molecular Systematics of Plants, P. S. Soltis, D. E. Soltis and J. J. Doyle, eds.(New York) London : Chapman and Hall. 1992, 36~49
Thompson J D , Higgins D G , Gibson T J . CLUSTAL W : improving the sensitivity of progressive multiple sequence alignment through equence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Research., 1994. 22, 4673- 4680.
Wang X-R. Genetic diversity and evolution of Eurasian Pinus species. T hesis. S LU Reprocentralen, Umeå, Sw eden, 1992
Wang X-R , Szmidt A E , Lu M-Z . Presence of chloroplast and mitochondrial DNA in pollen and female gametophytes and maternal inheritance of mitochondrial DNA in Pinus. Forest enetics., 1995. 3, 37- 44.
