Abstract:

Five structural polypeptides, with molecular weights of 120 kd, 116 kd, 110 kd, 66 kd and 33 kd, respectively, were found in SDS-PAGE profile of purified virus particles of Dendrolimus punctatus cytoplasmic polyhedrosis virus (DpCPV), Wenshan (Yunnan Province) strain. DpCPV polyhedron contains two major polypeptides of m.w. 30 kd and 28 kd. Four monoclonal antibodies, as 2D5, 2D10, 3D4 and 6B3, were prepared against purified DpCPV particles, and their subclasses were determined. These monoclonal antibodies were used in DpCPV detection by ELISA. Results obtained in vitro DpCPV replication in SFE-HA-8212 and SFE-HA-831 cells (ovarian cell lines from Helicoverpa armigera) showed that these cells were persistently infected, however, the release of virus particles into the culture medium was seldom demonstrated. In Western blotting, traces of viral antigens were detected in SFE-HA-831 cells 18 hours after DpCPV infection. Batches of Dendrolimus larvae collected from pine forests in Guangdong and Yunnan provinces were examined by the immunological detection technique described here. Results confirmed its efficacy in long-term monitoring of DpCPV in Dendrolimus control and its epidemics in nature.

Key words: Dendrolimus punctatus cytoplasmic polyhedrosis virus, Monoclonal antibody, Biological control