741杨器官、体细胞胚胎发生的细胞组织学及生理学的研究

Abstract

Abstract:

The yellow green hard callus is the best embryogenic callus, which obtained from the stem tip of Populus alba ×(P. davidiana ×P. simonii) ×P. tomentosa on the induced medium (MS+KT0. 1mg·L^-1 +2, 4-D 0.05mg·L^-1) after 10 days culture. Its formation frequeny of adventure buds, adventure roots and somatic embryos ane 100 %、19.4 % and 13.3 % respectively on the differentiated medium (MS + KT 0.3mg·L^-1). Org ans and somatic embryos are all derived from the surface layer of calli. The somatic embryogenesis is very similar with the zygote embryogenesis. The esterase isozymes and soluble protein bands of various calli are very different.

Key words: Populus alba × (P. davidiana × P. simonii) × P. tomentosa, Stem tip, Somatic embryo esterase isozyme, Soluble protein

Kejiu Du,Junbao Zheng,Zhenhua Xu,Jiquan Li,Jimmao Wang. STUDIES ON HISTOLOGY AND PHYSIOLOGY OF THE ORGANGENESIS AND SOMATIC EMBRYOGENESIS OF POPULUS ALBA × (P. DAVIDIANA × P. SIMONII) × P. TOMENTOSA IN VITRO[J]. Scientia Silvae Sinicae, 1998, 34(6): 99-104.

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References 16

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STUDIES ON HISTOLOGY AND PHYSIOLOGY OF THE ORGANGENESIS AND SOMATIC EMBRYOGENESIS OF POPULUS ALBA × (P. DAVIDIANA × P. SIMONII) × P. TOMENTOSA IN VITRO

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