用PCR扩增16S rDNA检测泡桐组培苗丛枝病类菌原体*

Scientia Silvae Sinicae ›› 1996, Vol. 32 ›› Issue (6): 569-573.

DETECTION OF MYCOPLASMALIKE ORGANISMS(MLO) OF THE PAULOWNIA WITCHES' BROOM TISSUE CULTURE BY A POLYMERASE CHAIN REACTION AMPLIFIING A SEQUENCE OF 16S rDNA

Jiangshan Li¹,Kaixuan Jin²,Yue Wang²,Yaoguo Xiong³,Bingsheng Qiu⁴

1. The reserch institute of forestry CAF Beijing 100091
2. The reserch institute of forest protection CAF Beijing 100091
3. The reserch institute of forestry CAF Beijing 100091
4. Institute of Microbilolgy Academia Sinica Beijing 100080

Received:1994-12-13 Online:1996-11-25 Published:1996-11-25

Abstract

Abstract:

An oligonucleotide primer set (PaF/PaR) was designed for polymerase chain reaction (PCR) amplification of an approximately 1.2kb fragment DNA from Paulownia Witches' broom(PaWB) MLO on the basis of 16S rRNA sequence from O-MLO. Amplification of a 1.2kb DNA fragment from PaWB-MLO-infected plants DNA in total nucleic acid extrats of the host plant Paulownia spp., but no 1.2kb DNA fragment was observed when healthy DNA was used as a template. The study showed that the PCR method was able todetect PaWB-MLO in as little as 9.4pg of total DNA from infected Paulownia tissue culture plantlets.

Key words: Paulownia witches' broom, MLO 16S rDNA, Universal primers, 1.2kb amplification fragment, PCR detection

Jiangshan Li,Kaixuan Jin,Yue Wang,Yaoguo Xiong,Bingsheng Qiu. DETECTION OF MYCOPLASMALIKE ORGANISMS(MLO) OF THE PAULOWNIA WITCHES' BROOM TISSUE CULTURE BY A POLYMERASE CHAIN REACTION AMPLIFIING A SEQUENCE OF 16S rDNA[J]. Scientia Silvae Sinicae, 1996, 32(6): 569-573.

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References 8

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DETECTION OF MYCOPLASMALIKE ORGANISMS(MLO) OF THE PAULOWNIA WITCHES' BROOM TISSUE CULTURE BY A POLYMERASE CHAIN REACTION AMPLIFIING A SEQUENCE OF 16S rDNA

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