Scientia Silvae Sinicae ›› 2022, Vol. 58 ›› Issue (2): 89-99.doi: 10.11707/j.1001-7488.20220210
• Research papers • Previous Articles Next Articles
Jingen Peng1,Jinyu Gong1,2,Yuhai Fan3,Hua Zhang1,Yinfeng Zhang1,2,Yuqing Bai4,Yanmei Wang2,Lijuan Xie1,*
Received:
2021-10-26
Online:
2022-02-25
Published:
2022-04-26
Contact:
Lijuan Xie
CLC Number:
Jingen Peng,Jinyu Gong,Yuhai Fan,Hua Zhang,Yinfeng Zhang,Yuqing Bai,Yanmei Wang,Lijuan Xie. Diversity of Soil Microbial Communities in Rhizosphere and Non-rhizosphere of Rhododendron moulmainense[J]. Scientia Silvae Sinicae, 2022, 58(2): 89-99.
Table 1
Basic characteristics of sampling plots of Wutong Mountain"
样地位置 Plot | 树龄 Tree age/a | 坡度 Slope/(°) | 坡向 Aspect | 海拔 Altitude/m | 经纬度 Longitude and latitude |
万花屏 Wanhuaping | 36 | 32 | 东北 Eastnorth | 523 | 114°11′53″E, 22°34′31″N |
小梧桐 Xiaowutong | 36 | 22 | 西北 Westnorth | 608 | 114°11′29″E, 22°34′23″N |
山顶管理处 Peak Management Office | 36 | 26 | 西北 Westnorth | 597 | 114°11′28″E, 22°34′15″N |
Table 2
Chemical properties of R. moulmainense rhizosphere and non-rhizosphere soil"
土壤类型 Soil style | 土壤含水量 Soil water content (%) | pH | 有机质 Organic carbon/(g·kg-1) | 全氮 TN/(g·kg-1) | 碱解氮 AN/(mg·kg-1) | 全磷 TP/(g·kg-1) | 速效磷 AP/(mg·kg-1) | 全钾 TK/(g·kg-1) | 速效钾 AK/(mg·kg-1) |
根际 R | 24.31±4.50a | 4.34±0.27b | 15.60±5.34a | 2.24±0.93a | 125.22±36.70a | 0.61±0.16a | 49.98±18.90a | 11.84±2.82a | 17.87±3.88b |
非根际 NR | 24.74±3.94a | 4.81±0.33a | 14.78±5.00a | 2.12±0.89a | 122.31±37.15a | 0.57±0.08a | 30.07±14.85b | 12.28±1.40a | 29.43±3.10a |
Table 3
The diversity of the bacterial and fungal communities in R. moulmainense rhizosphere and non-rhizosphere soil"
微生物 Microbial | 土壤类型 Soil style | OTU数目 OTUs | ACE指数 ACE | Chao1指数 Chao1 index | Simpson指数 Simpson index | Shannon指数 Shannon index |
细菌 Bacteria | R | 1 170.00±74.59a | 1 254.71±75.81a | 1 278.33±72.33a | 0.99±0.00a | 8.10±0.10a |
NR | 1 262.33±103.24a | 1 325.70±110.93a | 1 351.74±112.48a | 0.99±0.00a | 8.25±0.19a | |
真菌 Fungi | R | 1 100.33±11.59a | 1 118.58±16.50a | 1 131.52±26.19a | 0.95±0.01a | 6.66±0.13a |
NR | 1 103.33±7.77a | 1 115.22±8.89a | 1 122.65±8.94a | 0.95±0.00a | 6.85±0.02a |
Table 3
The species number of the bacterial and fungal communities in rhizosphere and non-rhizosphere soil"
微生物 Microbial | 土壤类型 Soil style | 门 Phylum | 纲 Class | 目 Order | 科 Family | 属 Genus | 种 Species |
细菌 Bacteria | R | 23 | 64 | 125 | 188 | 277 | 291 |
NR | 25 | 69 | 134 | 201 | 291 | 305 | |
真菌 Fungi | R | 8 | 29 | 77 | 180 | 352 | 520 |
NR | 8 | 29 | 77 | 180 | 353 | 518 |
Table 4
Abundance of different trophic mode in rhizosphere and non-rhizosphere soil"
营养类型 Trophic Mode | 主要OTU序列 The main OTU ID | 丰度 Abundance(%) | 根际/非根际 R/NR | |
根际 R | 非根际 NR | |||
共生营养型 Symbiotroph | OTU7、OTU10、OTU22 | 33.44 | 23.57 | 1.42 |
腐生-共生营养型 Saprotroph-symbiotroph | OTU6、OTU8、OTU157 | 6.48 | 7.63 | 0.85 |
腐生营养型 Saprotroph | OTU117、OTU12、OTU28 | 19.84 | 22.77 | 0.87 |
病理-共生营养型 Pathotroph-symbiotroph | OTU267、OTU181、OTU1476 | 0.76 | 0.79 | 0.96 |
病理-腐生-共生营养型 Pathotroph-saprotroph-symbiotroph | OTU190、OTU259、OTU378 | 0.25 | 0.34 | 0.74 |
病理-腐生营养型 Pathotroph-saprotroph | OTU199、OTU252、OTU588 | 33.83 | 38.02 | 0.89 |
病理营养型 Pathotroph | OTU11、OTU50、OTU51 | 5.39 | 6.86 | 0.79 |
白宇清. 毛棉杜鹃繁殖生物学与低海拔地区引种适应性研究.北京: 北京林业大学, 2017. | |
Bai Y Q . Reproductive biology of Rhododendron moulmainense Hook f. and its adaptability of introduction at low altitude areas. Beijing: Beijing Forestry University, 2017. | |
白宇清, 谢利娟, 王定跃, 等. 毛棉杜鹃对不同光照及土壤排水条件的生长及生理响应. 林业科学, 2016, 52 (1): 48- 54. | |
Bai Y Q , Xie L J , Wang D Y , et al. Influences of different light intensities and soil water drainage on branch increment and some physiological indexes in leaves of Rhododendron moulmainense. Scientia Silvae Sinicae, 2016, 52 (1): 48- 54. | |
郭辉, 唐卫平. 不同林龄华北落叶松根际与非根际土壤酶和土壤微生物研究. 生态环境学报, 2020, 29 (11): 2163- 2170. | |
Guo H , Tang W P . Enzyme activity and microbial community diversity in rhizosphere and non-rhizosphere of Larix principis-rupprechtii. Ecology and Environmental Sciences, 2020, 29 (11): 2163- 2170. | |
侯慧, 董坤, 杨智仙, 等. 连作障碍发生机理研究进展. 土壤, 2016, 48 (6): 1068- 1076. | |
Hou H , Dong K , Yang Z X , et al. Advance in mechanism of continuous cropping obstacle. Soils, 2016, 48 (6): 1068- 1076. | |
黄娜, 周苡, 吴晓妮, 等. 滇池流域富磷退化山区主要优势植物对土壤酶活性的影响. 水土保持研究, 2019, 26 (4): 171- 176. | |
Huang N , Zhou Y , Wu X N , et al. Effects of dominant species on soil enzyme activity in the phosphorus-enriched degraded mountain area in the lake dianchi watershed of southwestern China. Research of Soil and Water Conservation, 2019, 26 (4): 171- 176. | |
黄滔, 廖菊阳, 唐红. 毛棉杜鹃地理分布及其开发利用. 湖南环境生物职业技术学院学报, 2010, 16 (2): 1- 4. | |
Huang T , Liao J Y , Tang H , et al. Geographical distribution and utilization of Rhododendron moulmainense in China. Journal of Hunan Environment-Biological Polytechnic, 2010, 16 (2): 1- 4. | |
蒋治岩, 邹青勤, 杨柳, 等. 典型黑土区不同菌根类型树种根系分泌速率及根际效应差异. 生态学杂志, 2021, 40 (9): 2709- 2718. | |
Jiang Z Y , Zou Q Q , Yang L , et al. Root exudation rate and rhizosphere effect of different mycorrhizal associations of tree species in typical black soil area. Chinese Journal of Ecology, 2021, 40 (9): 2709- 2718. | |
姜子德, 戚佩坤, 陈永青, 等. 广州地区西洋杜鹃根腐与茎基腐病病原种类鉴定. 云南农业大学学报(自然科学版), 2000, (4): 333- 336. | |
Jiang Z D , Qi P K , Chen Y Q , et al. Identification of the roton roots and basal stem of Rhododendron spp. in Guangzhou area. Journal of Yunnan Agricultural University(Natural Science), 2000, (4): 333- 336. | |
李亚东, 郝瑞, 张大鹏. 越桔菌根真菌研究进展——文献综述. 园艺学报, 1996, (2): 133- 138. | |
Li Y D , Hao R , Zhang D P . Advances in research on mycorrhiza fungi of blueberry: a literature review. Acta Horticulturae Sinica, 1996, (2): 133- 138. | |
李岩, 何学敏, 杨晓东, 等. 不同生境黑果枸杞根际与非根际土壤微生物群落多样性. 生态学报, 2018, 38 (17): 5983- 5995. | |
Li Y , He X M , Yang X D , et al. The microbial community diversity of the rhizosphere and bulk soils of Lycium ruthenicum in different habitats. Acta Ecologica Sinica, 2018, 38 (17): 5983- 5995. | |
廖映辉, 黄彩微, 史佑海, 等. 海南霸王岭毛棉杜鹃根部真菌的多样性. 江苏农业科学, 2016, 44 (6): 432- 437. | |
Liao Y H , Huang C W , Shi Y H , et al. The fungi diversity in roots of the Rhododendron moulmainense in Hainan Bawangling. Jiangsu Agricultural Sciences, 2016, 44 (6): 432- 437. | |
刘光崧. 土壤理化分析与剖面描述.北京: 中国标准出版社, 1996: 123- 260. | |
Liu G S . Soil physical and chemical analysis & description of soil profiles.Beijing: Standards Press of China, 1996: 123- 260. | |
陆雅海, 张福锁. 根际微生物研究进展. 土壤, 2006, (2): 113- 121. | |
Lu Y H , Zhang F S . The advances in rhizosphere microbiology. Soils, 2006, (2): 113- 121. | |
马志良, 赵文强, 刘美, 等. 增温对高寒灌丛根际和非根际土壤微生物生物量碳氮的影响. 应用生态学报, 2019, 30 (6): 1893- 1900. | |
Ma Z L , Zhao W Q , Liu M , et al. Effects of warming on microbial biomass carbon and nitrogen in the rhizosphere and bulk soil in an alpine scrub ecosystem. Chinese Journal of Applied Ecology, 2019, 30 (6): 1893- 1900. | |
欧静, 刘仁阳, 陈训. 桃叶杜鹃菌根显微结构及侵染情况. 中南林业科技大学学报, 2012, 32 (11): 28- 33. | |
Ou J , Liu R Y , Chen X. , et al. Study on microstructure and infections of Rhododendron annae mycorrhiza. Journal of Central South University of Forestry & Technology, 2012, 32 (11): 28- 33. | |
任纬恒, 唐明, 王灵军, 等. 百里杜鹃保护区马缨杜鹃土壤真菌群落特征. 基因组学与应用生物学, 2020, 39 (3): 1172- 1177. | |
Ren W H , Tang M , Wang L J , et al. The characteristics of the soil fungus community of Rhododendron delavayi franch in the Baili Rhododendron Nature Reserve. Genomics and Applied Biology, 2020, 39 (3): 1172- 1177. | |
阮柳, 马占鸿, 刘振宇, 等. 苜蓿根腐病的病原分离、鉴定与杀菌剂毒力测定. 中国农业大学学报, 2016, 21 (6): 56- 67. | |
Ruan L , Ma Z H , Liu Z Y , et al. Isolation, identification and toxxicity determination of pathogens causing alfalfa root rot. Journal of China Agricultural University, 2016, 21 (6): 56- 67. | |
石柯, 董士刚, 申凤敏, 等. 小麦播量与减氮对潮土微生物量碳氮及土壤酶活性的影响. 中国农业科学, 2019, 52 (15): 2646- 2663. | |
Shi K , Dong S G , Shen F M , et al. Effects of wheat seeding rate with nitrogen fertilizer application reduction on soil microbial biomass carbon, nitrogen and enzyme activities in fluvo-aquic soil in Huang-Huai plain. Scientia Agricultura Sinica, 2019, 52 (15): 2646- 2663. | |
孙敏. 毛棉杜鹃花芽分化过程及某些生理特性的研究.呼和浩特: 内蒙古农业大学, 2009. | |
Sun M . Studies on differentiation process of flower buds of Rhododendron moulmainense Hook. and some physiological charactors. Huhehot: Inner Mongolia Agricultural University, 2009. | |
孙英杰, 徐广平, 沈育伊, 等. 桂林会仙喀斯特湿地芦苇群落区土壤酶活性. 湿地科学, 2018, 16 (2): 196- 203. | |
Sun Y J , Xu G P , Shen Y Y , et al. Soil enzyme activities of phragmites australis community area in Huixian karst wetland Gulin. Wetland Science, 2018, 16 (2): 196- 203. | |
童琪, 陈玫婷, 龙菁琦, 等. 不同龄组南酸枣根际与非根际土壤养分特征研究. 中南林业科技大学学报, 2019, 39 (12): 108- 113. | |
Tong Q , Chen M T , Long J Q , et al. Research on soil nutrient characteristics at rhizosphere and non-rhizosphere for different age groups of Choerospondias axillaris. Journal of Central South University of Forestry & Technology, 2019, 39 (12): 108- 113. | |
唐燕, 李敏, 马焕成, 等. 云南轿子山腋花杜鹃菌根多样性研究. 云南大学学报(自然科学版), 2019, 41 (5): 1062- 1072. | |
Tang Y , Li M , Ma H C , et al. Mycorrhizal diversity of Rhododendron racemosum Franch. in Yunnan Jiaozi Mountain. Journal of Yunnan University(Natural Sciences Edition), 2019, 41 (5): 1062- 1072. | |
王光华, RaaijmakersJos M.. 生防细菌产生的拮抗物质及其在生物防治中的作用. 应用生态学报, 2004, (6): 1100- 1104. | |
Wang G H , Jos M R . Antibiotics production by bacterial agents and its role in biological control. Chinese Journal of Applied Ecology, 2004, (6): 1100- 1104. | |
熊丹, 欧静, 李林盼, 等. 黔中地区马尾松林下杜鹃根部内生真菌群落组成及其生态功能. 生态学报, 2020, 40 (4): 1228- 1239. | |
Xiong D , Ou J , Li L P , et al. Community composition and ecological function analysis of endophytic fungi in the roots of Rhododendron simsii in Pinus massoniana forest in central Guizhou. Acta Ecologica Sinica, 2020, 40 (4): 1228- 1239. | |
张艳华, 孙立夫. 杜鹃花科植物菌根的研究进展. 菌物学报, 2021, 40 (6): 1299- 1316. | |
Zhang Y H , Sun L F . Research advances on the mycorrhizas of Ericaceae plants. Mycosystema, 2021, 40 (6): 1299- 1316. | |
赵富群, 尹茜, 洪文君, 等. 毛棉杜鹃的组织培养与快速繁殖. 植物生理学报, 2017, 53 (9): 1666- 1672. | |
Zhao F Q , Yi X , Hong W J , et al. Tissue culture and rapid propagation of Rhododendron moulmainense. Plant Physiology Journal, 2017, 53 (9): 1666- 1672. | |
郑梅霞, 陈宏, 朱育菁, 等. 七叶一枝花根际与非根际土壤细菌群落多样性. 福建农业学报, 2020, 35 (12): 1357- 1367. | |
Zheng M X , Chen H , Zhu Y J , et al. Microbial diversity in rhizosphere and non-rhizosphere soils of Paris polyphylla var. chinensis Plants. Fujian Journal of Agricultural Sciences, 2020, 35 (12): 1357- 1367. | |
郑钰, 高博, 孙立夫, 等. 银叶杜鹃和繁花杜鹃根部真菌的多样性. 生物多样性, 2010, 18 (1): 76- 82. | |
Zheng J , Gao B , Sun L F , et al. Diversity of fungi associated with Rhododendron argyrophyllum and R. floribundum hair roots in Sichuan, China. Biodiversity Science, 2010, 18 (1): 76- 82. | |
周玉洁, 庄雪影, 洪文君, 等. 不同苗龄毛棉杜鹃幼苗与杜鹃类菌根真菌的共生效应. 南方农业学报, 2017, 8 (8): 1458- 1464. | |
Zhou Y J , Zhuang X Y , Hong W J , et al. Symbiotic effects between Rhododendron moulmainense seedlings of different ages and ericoid mycorrhizal fungi. Journal of Southern Agriculture, 2017, 8 (8): 1458- 1464. | |
字洪标, 向泽宇, 王根绪, 等. 青海不同林分土壤微生物群落结构(PLFA). 林业科学, 2017, 53 (3): 21- 32. | |
Zi H B , Xiang Z Y , Wang G X , et al. Profile of soil microbia community under different stand types in Qinghai province. Scientia Silvae Sinicae, 2017, 53 (3): 21- 32. | |
Mousavi S M , Motesharezadeh B , Hosseini H M , et al. Root-induced changes of Zn and Pb dynamics in the rhizosphere of sunflower with different plant growth promoting treatments in a heavily contaminated soil. Ecotoxicology and environmental safety, 2018, 147, 206- 216. | |
Nguyen , Song Z , Bates S T , et al. FUNGuild: An open annotation tool for parsing fungal community datasets by ecological guild. Fungal Ecology, 2016, 20, 241- 248. | |
Shi S J , Nuccio E , Herman D J , et al. Successional trajectories of rhizosphere bacterial communities over consecutive seasons. mBio, 2015, 6 (4): 746- 761. | |
Surette M A , Sturz A V , Lada R R , et al. Bacterial endophytes in processing carrots (Daucus carota L. var. sativus): their localization, population density, biodiversity and their effects on plant growth. Plant and Soil, 2003, 253 (2): 381- 390. | |
Wang X J , Peng C Y , Liang J S , et al. The complete chloroplast genome of Paris Polyphylla var. chinensis, an endemic medicinal herb in China. Mitochondrial DNA Part B, 2019, 4 (2): 3888- 3889. | |
Yang S , Xing S , Liu C , et al. Effects of root pruning on the vegetative growth and fruit quality of Zhanhuadongzao trees. Horticultural Science, 2010, 37, 14- 21. |
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