Welcome to visit Scientia Silvae Sinicae,Today is

Scientia Silvae Sinicae ›› 2022, Vol. 58 ›› Issue (2): 89-99.doi: 10.11707/j.1001-7488.20220210

• Research papers • Previous Articles     Next Articles

Diversity of Soil Microbial Communities in Rhizosphere and Non-rhizosphere of Rhododendron moulmainense

Jingen Peng1,Jinyu Gong1,2,Yuhai Fan3,Hua Zhang1,Yinfeng Zhang1,2,Yuqing Bai4,Yanmei Wang2,Lijuan Xie1,*   

  1. 1. School of Architecture and Engineering, Shenzhen Polytechnic Shenzhen 518055
    2. College of Forestry, Henan Agricultural University Zhengzhou 450002
    3. Rizhao Natural Resources and Planning Bureau Rizhao 276827
    4. Administrative Office of Wutong Mountain National Park Shenzhen 518004
  • Received:2021-10-26 Online:2022-02-25 Published:2022-04-26
  • Contact: Lijuan Xie


Objective: This study aims to study the spatial structure of soil microbial communities and the physicochemical properties of soil in rhizosphere and non rhizosphere of Rhododendron moulmainense in Shenzhen Wutong Mountain, so as to provide theoretical support for the further study of the rhizosphere microecology of R. moulmainense. Method: The rhizosphere (R) and non rhizosphere (NR) soil of R. moulmainense were used as the research object, the soil physical and chemical indexes were measured, and the community characteristics of soil bacteria and fungi were analyzed by high-throughput sequencing technology. Results: The pH of rhizosphere and non-rhizosphere soil was slightly acidic, and the rhizosphere had significantly lower pH than non-rhizosphere (P < 0.05). The available phosphorus in rhizosphere soil was significantly higher than that in non-rhizosphere soil (P < 0.05).The organic matter, total nitrogen and alkali-hydrolyzed nitrogen in rhizosphere soil were higher than those in non-rhizosphere soil, but there was no significant difference. A total of 23 phyla and 291 species of rhizosphere bacteria and 25 phyla and 305 species of non-rhizosphere bacteria were annotated respectively. The rhizosphere fungi were annotated to 8 phyla and 520 species, and the non rhizosphere fungi were annotated to 8 phyla and 518 species. The dominant bacteria in rhizosphere and non rhizosphere were Acidobacteria, Protecbacteria, Actinobacteria, Chloroflexi, etc. The dominant fungi were Ascomycota, Basidiomycota and Mortierellomycota. Rhizosphere and non-rhizosphere dominant bacteria of R. moulmainense were mainly subgroup_2, Acidobacteriales, solidobacterales, rhizobiales, etc. The dominant fungi in rhizosphere and non rhizosphere were mainly Eurotiales, Hypocreales, Sebacinales, etc.There was no difference in the diversity index of bacteria and fungi between rhizosphere and non-rhizosphere, while Beta diversity analysis showed that there were differences in the diversity of soil bacterial and fungal community structure between rhizosphere and non-rhizosphere. The results of functional prediction analysis based on FUNGuild showed that the functions of rhizosphere and non-rhizosphere fungi were mainly Symbiotroph trophic(23.57%~33.44%), Saprotroph-Symbiotroph trophic(6.48%~7.63%), Saprotroph(19.84%~22.77%), Pathotroph-Symbiotroph trophic(0.76%~0.79%), Pathotroph-Saprotroph-Symbiotroph trophic(0.25%~0.34%), Pathotroph-Saprotroph(33.83%~38.02%)and Pathotroph trophic(5.39%~6.86%). Both rhizosphere and non-rhizosphere soils were dominated by pathological saprophytic and symbiotic vegetative fungi. Conclusion: The rhizosphere and non-rhizosphere soils are slightly acidic. There is no significant difference in the species of dominant microorganisms between rhizosphere and non-rhizosphere.The dominant bacteria are Acidobacteria and Proteobacteria, and the dominant fungi are Ascomycota and Basidiomycota. There are differences in fungal functional structure between rhizosphere and non-rhizosphere, mainly because rhizosphere symbiotic fungi are much higher than non-rhizosphere fungi.

Key words: Rhododendron moulmainense, physicochemical properties of soil, Soil microbial diversity, Symbiotic trophic type

CLC Number: