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Scientia Silvae Sinicae ›› 2008, Vol. 44 ›› Issue (4): 60-68.doi: 10.11707/j.1001-7488.20080412

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Isolation and Identification of the Pathogen That Causes Mosaic on Periwinkle

Cao Yanyong1,Shi Yan1,Fan Zaifeng1,Song Limin2,Tian Guozhong2   

  1. (1. Key Lab. for Molecular Plant Pathology of the Ministry of Agriculture of PRC Department of Plant Pathology, China Agricultural University Beijing 100094; 2. Research Institute of Forest Ecology, Environment and Protection, CAF Key Lab. of Forest Protection， State Forestry Administration Beijing 100091)
  • Received:2007-02-09 Revised:1900-01-01 Online:2008-04-25 Published:2008-04-25

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Abstract:

The systemic mosaic and malformation symptoms were observed on the leaves of periwinkle (Catharanthus roseus) from Bo’ao, Qionghai, Hainan Province and Xinghai Park, Guangzhou, Guangdong Province. With the infected tissue culture, host range determination, serological assay and viral particle morphological observation, dsRNA analysis, the pathogen was identified to be cucumber mosaic virus (CMV). Cloning of the coat protein gene and analysis of the nucleotide sequences further demonstrated that this CMV isolate was tentatively assigned to the IB subgroup of CMV.

Key words: Catharanthus roseus, mosaic disease, cucumber mosaic virus（CMV）